Dataset: Measurements of treatment tub seawater conditions: pH and total alkalinity

Name: pH and total alkalinity measurements of treatment tub seawater during lab experiments conducted in spring 2022.
Published: 2024-03-24
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.922037.1

Laboratory at the University of Pennsylvania

Temporal Extent: 2022-01-26 - 2022-05-02

Project:

Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)

Principal Investigator:

Katie Barott (University of Pennsylvania, Penn)

Scientist:

Kristen Brown (University of Pennsylvania, Penn)

Kelsey Speer (University of Pennsylvania, Penn)

Student:

Benjamin Glass (University of Pennsylvania, Penn)

Angela Schmitt (University of Pennsylvania, Penn)

Contact:

Benjamin Glass (University of Pennsylvania, Penn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-03-24

Restricted:

Validated:

Yes

Current State:

Final no updates expected

pH and total alkalinity measurements of treatment tub seawater during lab experiments conducted in spring 2022.

Abstract:

Ocean acidification (OA) resulting from anthropogenic CO2 emissions is impairing the reproduction of marine organisms. While parental exposure to OA can protect offspring via carryover effects, this phenomenon is poorly understood in many marine invertebrate taxa. We examined how parental exposure to acidified (pH 7.40) versus ambient (pH 7.72) seawater influenced reproduction and offspring performance across six gametogenic cycles (13 weeks) in the estuarine sea anemone Nematostella vectensis. This dataset contains data resulting from measurements of treatment tub seawater conditions, including pH and total alkalinity.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2024-03-24	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.922037.1	2024-03-24

Description

Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).

Methods & Sampling

Nematostella vectensis (Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).

A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).

All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals^Ⓡ reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.

Data Processing Description

Salinity, pH, and temperature were measured and recorded approximately daily to ensure the maintenance of experimental conditions. Seawater pH was measured and recorded using a handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA), which was calibrated once a week using calibration solutions (pH 7 and 10) supplied by the probe manufacturer. Salinity and temperature were measured and recorded using a handheld meter (YSI Incorporated, Yellow Springs, OH, USA), and salinity was adjusted with deionized (DI) water as needed.

Duplicate 50 mL seawater samples were collected from each tub every three days, then immediately sterilized with a 0.22 μm syringe filter (Sigma-Aldrich, Burlington, MA, USA) and stored at 4°C in conical tubes (Corning, Corning, NY, USA) until processing for total alkalinity (TA). TA was determined via titration using a Metrohm 905 Titrando (Metrohm, Herisau, Switzerland). Parameters of the carbonate system in the seawater samples [e.g. (carbonate), (bicarbonate), aragonite saturation state] were calculated from temperature, salinity, TA, and pH using the seacarb package in R.

BCO-DMO Processing

* Adjusted parameter names to comply with database requirements

Data Files

File
922037_v1_seawater.csv (Comma Separated Values (.csv), 6.48 KB) MD5:3c671ab3e2ef119c8ea924906c9ba924 Primary data file for dataset ID 922037, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1923743

Deployments

None available yet

Instruments

Metrohm 905 Titrando potentiometric titrator

Supplied Name: Metrohm 905 Titrando (Metrohm, Herisau, Switzerland)

Supplied Description:

Metrohm 905 Titrando (Metrohm, Herisau, Switzerland) for total alkalinity determination

Instrument Type

Generic Name: Metrohm 905 Titrando potentiometric titrator

Generic Description:

The Metrohm 905 Titrando potentiometric titrator is a modular potentiometric titrator for dynamic, monotonic, and set endpoint titrations. The device includes magnetic stirrers, rod stirrers, and a titration stand. It can be connected to various dosing units which include a buret and are attached to the reagent. Operation is carried out by means of a touch-sensitive display or with high-performance PC software. Temperature is measured by a Pt1000 or NTC. Ranges of the outputs are -13 to 20 pH, -1200 to 1200 mV, and -150 to 250 deg. C (Pt1000) or -5 ro 250 deg. C (NTC). Resolutions of the outputs are 0.001 for pH, 0.1 for mV, 0.1 deg. C for temperature. The measuring interval is of 100 ms. Works in conditions from 5 to 45 deg. C and at a maximum of 80 % relative humidity.

pH Sensor

Supplied Name: Handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA)

Supplied Description:

Handheld pH glass electrode (Mettler Toledo, Columbus, OH, USA), calibrated once a week using calibration solutions (pH 7 and 10) supplied by the probe manufacturer

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Water Quality Multiprobe

Supplied Name: Handheld meter (YSI Incorporated, Yellow Springs, OH, USA

Supplied Description:

Handheld meter (YSI Incorporated, Yellow Springs, OH, USA) for salnity and temperature, and salinity was adjusted with deionized (DI) water as needed

Instrument Type

Generic Name: Water Quality Multiprobe

Generic Description:

An instrument which measures multiple water quality parameters based on the sensor configuration.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date on which seawater samples were collected from experimental tubs Format: %Y-%m-%d	unitless	date
Treatment	Experimental treatment into which anemones were placed (ambient or acidic seawater pH)	unitless	treatment
pH	Seawater pH	Arbitrary on pH scale	pH
mV	Conductivity of seawater in millivolts	millivolts (mV)	cond
Salinity	Seawater salinity	unitless	sal
Temperature	Temperature of seawater	Degrees Celsius	temperature
TA_1	Total alkalinity of sample replicate 1	umol/kg	TALK
TA_2	Total alkalinity of sample replicate 2	umol/kg	TALK
TA_average	Average total alkalinity of sample replicates	umol/kg	TALK
TA	Conversion of TA average into mol/kg	mol/kg	TALK
Alkalinity	Conversion of TA mol/kg	mol/kg*10^-3	TALK