Dataset: Synechococcus Growth on DOP Experiments - IVF
Data Citation:
Duhamel, S., Diaz, J., Djaoudi, K., Waggoner, E. (2024) Laboratory-cultured Synechococcus (WH8102 and WH5701) growth (vivo fluorescence) on dissolved organic phosphorus (DOP) from experiments between 2018-2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-06-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.929212.1 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
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DOI:10.26008/1912/bco-dmo.929212.1
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Laboratory experiments at the University of Arizona, Tucson, Arizona, US
Temporal Extent: 2018 - 2023
Principal Investigator:
Solange Duhamel (University of Arizona, UA)
Co-Principal Investigator:
Julia Diaz (University of California-San Diego, UCSD-SIO)
Scientist:
Kahina Djaoudi (University of Arizona, UA)
Emily Waggoner (University of Arizona, UA)
BCO-DMO Data Manager:
Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2024-06-03
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Laboratory-cultured Synechococcus (WH8102 and WH5701) growth (vivo fluorescence) on dissolved organic phosphorus (DOP) from experiments between 2018-2023
Abstract:
Laboratory culture Synechococcus (WH8102 and WH5701) growth on dissolved organic phosphorus (DOP). These data were collected as part of a study of "Dissolved organic Phosphorus bond-class utilization by Synechococcus" (Waggoner et al. 2024).
Study Abstract:
Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.