Dataset: Field measurements of periostracum cover from focal population

Name: Field measurements of periostracum cover of mussels (Mytilus californianus) from focal population at Marshall Gulch Beach, CA in July and August 2022
Published: 2024-12-26
Keywords: periostracum, mussel, microhabitat, biota

Cite This Dataset

DOI:10.26008/1912/bco-dmo.935484.1

Spatial Extent: N:38.369738 E:-123.073921 S:38.369738 W:-123.073921

Marshall Gulch Beach, California

Temporal Extent: 2022-07-17 - 2022-08-18

Project:

Invertebrate calcification and behavior in seawater of decoupled carbonate chemistry (OA decoupling)

Principal Investigator:

Brian Gaylord (University of California - Davis: Bodega Marine Laboratory, UC Davis-BML)

Student:

Alisha Saley (University of California - Davis: Bodega Marine Laboratory, UC Davis-BML)

BCO-DMO Data Manager:

Lynne M. Merchant (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-12-26

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Field measurements of periostracum cover of mussels (Mytilus californianus) from focal population at Marshall Gulch Beach, CA in July and August 2022

Abstract:

These data build off of experimental incubations described in Dataset 1 and 2. To contextualize laboratory incubations, we measured periostracum cover of live California mussels from multiple microhabitats (relative tidal height and degree of sun exposure) in our focal sample population. This dataset represents periostracum cover measurements of California Mussels, Mytilus californianus, conducted at Marshall Gulch Beach, CA in July and August 2022. Dataset 1: Lab incubations of mussels (Mytilus californianus) examining the influence of periostracum cover and pH on external shell dissolution at Marshall Gulch Beach, CA from August 2021 to March 2022 (see BCO-DMO related dataset) Dataset 2: Lab incubations of mussels (Mytilus californianus) examining the influence of periostracum cover and pH on external shell dissolution at Marshall Gulch Beach, CA from August 2021 to March 2022 (see BCO-DMO related dataset)

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2024-12-26	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.935484.1	2024-12-29

Methods & Sampling

To provide ecological context for the laboratory dissolution experiments, we also measured the percent cover of periostracum of living California Mytilus californianus mussels in the field, focusing on mussels inhabiting different microhabitats within the intertidal zone. We sampled mussels across two tidal heights and three levels of sun exposure on 17 July and 18 August 2022 from the same population (Marshall Gulch Beach, California) where we had collected mussels for the laboratory trials (see the related BCO-DMO datasets). We sampled from the topmost layer of mussels within a 20 cm x 20 cm quadrat positioned within the center of each of 8 mussel beds at each tidal height (characterized as ‘high’ or low’, -0.3 – +0 m sea level height (SLH) and +0.3 - +1 m SLH), haphazardly choosing the first 20 adult individuals within a pre-selected size range (n = 113 mussels total, 54 mm + 4 mm SD). We characterized sun exposure based on the cardinal direction of the mussel bed orientation, which included mussels oriented 200° – 240° SW (termed ‘intense’), 30° – 70° NE (termed ‘average’), and 300° – 340° NW (termed ‘shade’). We photographed both valves of each mussel, ensuring a scale bar was visible in the field of view for use in ImageJ, and averaged the coverage of periostracum across the two valves as the metric of percent cover for a given individual.

Data Processing Description

Data analysis:

We performed computations with R statistical software, RStudio version 2023.06.2. We used a two-way ANOVA to compare average periostracum cover in adult mussels collected from the field at two relative tidal heights and three levels of sun exposure. We omitted the interaction term between the treatments after confirming it failed to explain significant variability in the model. Using tidal height and sun exposure as categorical predictors to explain periostracum cover, we then ran a TukeyHSD test (95% confidence) to identify which microhabitat types differed from one another via post-hoc, pairwise comparisons.

BCO-DMO Processing

1) Processed the submitted file named ave.field_PUB.csv with the BCO-DMO tool Laminar.

BCO-DMO parameter naming convention is for names to only include alphanumeric characters and underscores. Periods in the parameter names have been replaced with underscores.

peri.sa renamed to peri_percent to clarify it represents a percent.

shell.l renamed to shell_L_avg to clarify it's an average

sa.tot renamed to shell_A_avg to clarify it's an average

Saved dataset to primary data file named 935484_v1_measurements_of_mussels_periostracum_cover.csv

2) Checked species taxonomy of mussel collected, Mytilus californianus, and created a taxonomy file using information from the World Register of Marine Species (WoRMS: marinespecies.org) with columns: ScientificName, AphiaID, LSID, Authority, Kingdom, Phylum, Class, Order, Family, Genus, Species

Data Files

File
935484_v1_measurements_of_mussels_periostracum_cover.csv (Comma Separated Values (.csv), 11.28 KB) MD5:bcbf09663cc0aa1dac19ac7b58dad6d0 Primary data file for dataset ID 935484, version 1

Supplemental Files

File

Species taxonomy
filename: species_taxonomy.csv

(Comma Separated Values (.csv), 240 bytes)
MD5:6adb15fd7f2eba705b30b09b7a827689

Species taxonomy from the World Register of Marine Species (WoRMS: marinespecies.org) with columns: ScientificName, AphiaID, LSID, Authority, Kingdom, Phylum, Class, Order, Family, Genus, Species

Column descriptions

ScientificName: Genus and Species name
AphiaID: Unique taxonomic identifier at the World Register of Marine Species (WoRMS: marinespecies.org)
LSID: The Life Sciences Identifier (LSID) is an Interoperable Informatics Infrastructure Consortium (I3C) and OMG Life Sciences Research (LSR) Uniform Resource Name (URN) specification in progress.
Authority: The taxonomic authority is the surname of the person who first described the species

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-2129942

Deployments

None available yet

Instruments

Camera

Supplied Name: camera

Supplied Description:

Instrument Type

Generic Name: Camera

Acronym: camera

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/311/

Generic Description:

All types of photographic equipment including stills, video, film and digital systems.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
ID	Shell ID: identification number of individual mussel valve	unitless	sample
species	Mussel species	unitless	species
lat	latitude, South is negative	deicmal degrees	lat
lon	longitude, West is negative	decimal degrees	lon
date_start	collection start date Format: %Y-%m-%d	unitless	date_start
date_end	collection end date Format: %Y-%m-%d	unitless	date_end
light	Microhabitat: sun exposure based on the cardinal direction of the mussel bed orientation, which included mussels oriented 200° – 240° SW (termed ‘intense’), 30° – 70° NE (termed ‘average’), and 300° – 340° NW (termed ‘shade’): N (shady), M (average), or F (intense)	unitless	light_intensity
height	Microhabitat: tidal height: tidal height characterized as ‘high’ (HT) or low’ (LT) based on measured sea level height (-0.3 – +0 m sea level height (SLH) and +0.3 - +1 m SLH)	unitless	tide
peri_percent	Periostracum cover: percent cover of intact periostracum over mussel valve	percentage (%)	no_bcodmo_term
shell_L_avg	Valve length: average value between longest measured length of both mussel valves (ImageJ)	milimeter (mm)	length
shell_A_avg	Shell area: average mussel valve area measurement between both mussel valves (ImageJ)	milimeters squared (mm^2)	area_measured

Status message

Database

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Dataset: Field measurements of periostracum cover from focal population