Dataset: Particulate metals from the scandium incubation experiment on PUPCYCLE I in the California Current System in 2019

Name: Particulate metals from a scandium incubation experiment during the PUPCYCLE I R/V Oceanus cruise 1905B in the California Current System in 2019
Published: 2024-10-10
Keywords: Incubation, Scandium, Iron, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.940088.1

Spatial Extent: N:35.92333 E:-121.54 S:35.92333 W:-121.54

California Current System, just offshore the Big Sur coast, 35°55.4’N, 121°32.4’W

Temporal Extent: 2019-06-02

Project:

CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans (Upwelled Phytoplankton Dynamics)

Principal Investigator:

Matthew P. Hurst (California Polytechnic State University Humboldt, Cal Poly Humboldt)

Adrian Marchetti (University of North Carolina at Chapel Hill, UNC-Chapel Hill)

Claire P. Till (California Polytechnic State University Humboldt, Cal Poly Humboldt)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-10-10

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Particulate metals from a scandium incubation experiment during the PUPCYCLE I R/V Oceanus cruise 1905B in the California Current System in 2019

Abstract:

We performed an incubation experiment with added dissolved scandium and/or iron in waters sampled in the California Current System during the PUPCYCLE I cruise in 2019 with Chief Scientist Adrian Marchetti. PUPCYCLE I (Phytoplankton response to the UPwelling CYCLE) took place in summer 2019 onboard the R/V Oceanus (OC 1905b). Water for the incubation was collected from 15 m just off the Big Sur coast 2 June 2019. This was in a region with an extremely narrow shelf. There were five total treatments run in triplicate: control (no addition), +5 nmol/kg dissolved Fe, +5 nmol/kg dissolved Sc, +5 nmol/kg dissolved Fe and +5 nmol/kg dissolved Sc, and filtered seawater with +5 nmol/kg dissolved Fe and +5 nmol/kg dissolved Sc. After 24 hours incubating, the incubation was harvested and analyzed for chlorophyll, nutrients, and dissolved and particulate Fe and Sc concentrations. The effort was to investigate similarities and differences in the oceanic chemical cycling of Fe and Sc.

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2024-10-10	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.940088.1	2024-10-24

Description

See the "Related Datasets" section for other datasets from this experiment.

Methods & Sampling

Sampling:

Seawater for the incubation was collected from ~15m at 35°55.4’N, 121°32.4’W using acid-cleaned HDPE tubing taped directly to the amsteel blue line. A lead weight coated with fiberglass and epoxy paint was at the bottom of the line. The pump was a teflon Wilden air-operated double-diaphragm pump. The tubing was run into a trace metal clean positive pressure bubble, where it was homogenized in acid-cleaned 50 gallon plastic barrels. From there, 4L cubitainers for the different treatments were rinsed and filled with the homogenized seawater, and then spiked appropriately based on treatment. These cubitainers had been pre-cleaned using the methods of Crawford et al. 2003.

The incubation setup:

We had five different treatments, with three replicates each:
control: no addition
+Fe: 5 nmol/kg added dissolved Fe
+Sc: 5 nmol/kg added dissolved Sc
+Fe and +Sc: 5 nmol/kg each of dissolved Sc and Fe added
filtered +Fe and + Sc: the seawater was filtered with 0.2 micrometer pre-cleaned supor Acropak filter before rinsing and filling the cubitainer, and then spiking with 5 nmol/kg each of dissolved Sc and Fe.

The cubitainers were incubated in an on-deck plexiglass incubator that was surface-seawater chilled and covered with a screening to achieve 30% of the incident irradiance. After 24 hours, the incubation was ended and each treatment was harvested.

Sample analysis:

Samples for labile particulates were sampled and analyzed by the Hurst lab. The cubitainers were sampled into 2 L polycarbonate bottles with three rinses before filling. They were filtered immediately through pre-cleaned 0.2 mm pore-size polycarbonate track-etched (PCTE) membrane filters (47 mm dia., Nuclepore, Whatman) mounted in Teflon filter sandwiches (Millipore). Filters were stored in the freezer before digestion with a Berger et al. (2008) leach (acetic acid and hydroxylamine), used to extract the labile particulates. Extracts were analyzed for Fe and Sc on the Thermo Fisher Element 2 extended range ICP-MS at UC Santa Cruz.

BCO-DMO Processing

* Sheet 1 of submitted file "May 2019 Sc incubation particulates for BCO-DMO.xlsx" was imported into the BCO-DMO data system for this dataset.
** In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.

* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]

Data Files

File
940088_v1_ scandium-inc-exp-p-metals.csv (Comma Separated Values (.csv), 1.13 KB) MD5:2f54c0326be8980b916b23c36d9c0f3f Primary data file for dataset ID 940088, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1751805

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
OC1905B	PUPCYCLE I	2019-05-24	R/V Oceanus	Adrian Marchetti (Chief Scientist)	data info

Instruments

Inductively Coupled Plasma Mass Spectrometer

Supplied Name: Thermo Element XR

Supplied Description:

Trace metal extracts were analyzed with a Thermo Element XR magnetic sector inductively coupled plasma mass spectrometer (ICP-MS)

Instrument Type

Generic Name: Inductively Coupled Plasma Mass Spectrometer

Acronym: ICP Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/

Generic Description:

An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Label	The name of each sample	unitless	sample
Timepoint	Timepoint (hours). how long the particular sample incubated for (24 hours in most cases; 0 hours for measurements of the initial conditions before the incubation)	hours	time_elapsed
Treatment	Description of the treatment for the particular sample	unitless	treatment
Replicate	There were three replicates for each treatment. This parameter serves to disambiguate them.	unitless	replicate
leachable_particulate_Fe	Concentration of iron in particles greater than 0.2 micrometers that were leachable with the weak acid leach	nanomoles per kilogram (nmol/kg)	Fe
leachable_particulate_Sc	Concentration of scandium in particles greater than 0.2 micrometers that were leachable with the weak acid leach	picomoles per kilogram (pmol/kg)	Sc

Status message

Database

Contribute Data

Dataset: Particulate metals from the scandium incubation experiment on PUPCYCLE I in the California Current System in 2019