Dataset: EPR 2019 Nutrients

Name: Dissolved nitrate plus nitrite, nitrite and phosphate from water column of the East Pacific Rise in April 2019 aboard the R/V Atlantis cruise AT42-09
Published: 2025-02-04
Keywords: Oxygen deficient zone, Nutrients, (2965), oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.948718.1

Spatial Extent: N:9.8333 E:-104.3023 S:9.8333 W:-104.3023

Water column of the Eastern Tropical North Pacific Ocean overlying the East Pacific Rise hydrothermal vent field.9°50'N 104°18.14'W Full depth profile to 2500 m

Temporal Extent: 2019-04-09 - 2022-09-15

Project:

Collaborative Research: From hot to cold in the dark - shifts in seafloor massive sulfide microbial communities as physical and geochemical conditions change after venting ceases (Hot2cold Vents)

Principal Investigator:

Jacob A. Cram (University of Maryland Center for Environmental Science, UMCES/HPL)

Scientist:

Clara Fuchsman (University of Maryland Center for Environmental Science, UMCES/HPL)

Jason Brent Sylvan (Texas A&M University, TAMU)

Benjamin Tully (University of Southern California, USC)

Student:

Paulina Huanca Valenzuela (University of Maryland Center for Environmental Science, UMCES/HPL)

Technician:

Jerry Frank (University of Maryland Center for Environmental Science, UMCES)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2025-02-04

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Dissolved nitrate plus nitrite, nitrite and phosphate from water column of the East Pacific Rise in April 2019 aboard the R/V Atlantis cruise AT42-09

Abstract:

These are dissolved inorganic nutrient concentrations from the eastern tropical north pacific ocean from the water column overlying the east pacific rise at 9°50'N 104°18.14'W including samples in hypoxic water. The measured nutrients are nitrate plus nitrite, nitrite, and phosphate. Samples were collected through two casts, one each on April 9 2019 and April 13 2019 by Dr. Benjamin Tully aboard the RV Atlantis cruise AT 42-09. Samples were run by Chesapeake Bay Laboratory's Analytical services facility following EPA method 353.2 with analysis overseen by Jerry Frank. While the cruise primarily focused on the hydrothermal vent field below, our team focused on measurements of suspended particles in the water column and their microbial communities. These nutrient data supported this analysis. These data support a paper in review titled "Quantitative microbial taxonomy across particle size, depth, and oxygen concentration" lead by Paulina Huanca.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2025-02-04	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.948718.1	2025-02-18

Description

These data were supported by NSF award OCE-1756339 and Horn Point Laboratory startup funds.

Results publication in review:
Fuchsman, C.A. and Cram, J.A. (n.d.) Size fractionated suspended organic carbon and nitrogen from the offshore Eastern Tropical North Pacific Oxygen Deficient Zone suggest contributions of picocyanobacteria and vertically migrating metazoans to organic matter. Global Biogeochemical Cycles. In review.
*preprint available at ESS Open Archive (Fuchsman & Cram 2024, doi:10.22541/essoar.173046855.50289201/v1)

Huanca, P., C.A. Fuchsman, B.J. Tully, J.B. Sylvan, J.A. Cram (n.d.) Quantitative microbial taxonomy across particle size, depth, and oxygen concentration. Frontiers in Microbiology. In review.

Methods & Sampling

[See "Related Publications" section for full references and links to methods and standards cited here.]

Water was collected from the CTD rosette and prefiltered through a 0.2 μm Sterivex filter into a triplle rinsed 50 ml falcon tube. Samples were stored at -80 °C, shipped to University of Southern California on dry ice, stored for one year, and then shipped on to UMCES. At UMCES prefiltered (0.2 µm) dissolved nitrate, nitrite, and phosphate from the water samples were analyzed at the Nutrient Analytical Services Laboratory at the Chesapeake Biological Laboratory (CBL). The ELAP Certification Number for all samples is 12066 Methods for each nutrient follow:

Nitrite plus nitrate:

Measured by using the Method EPA 353.2 - CADMIUM (in the case of samples AT4209-007-Surf, and AT2309-007-Chla-Top) and method ASTDM D-7781-14 for all other samples.

Method detection limit was 0.0004 for method EPA 353.2 - CADMIUM and 0.0057 for method ASTM D-7781-14. Reporting limit was 0.0056 for method EPA 353.2 - CADMIUM and 0.028 for method ASTM D-7781-14.

Nitrate with the method EPA 353.2

Method detection limit was 0.0009 and reporting limit was 0.0032

Phosphate by using method EPA 365.1

Method detection limit was 0.0034 and reporting limit was 0.0102

Problems/Issues

All samples were stored from spring 2019 though fall 2022 longer than 28 days from collection to sample processing recommended by the sequencing center.

Data Processing Description

Data were processed by Chesapeake Bay Laboratory Analytical Services using their standard pipeline. Final data files for upload to BCO DMO were curated by hand by Jacob Cram.

BCO-DMO Processing

Data Version 1:
* data within the submitted file "EPR_Nutrients_for_BCODMO_W.csv", was imported into the BCO-DMO data system for this dataset. Several revisions of this file were uploaded, but the version that corresponds to public version 1 of this dataset "EPR_Nutrients_for_BCODMO_W.csv" was uploaded 2025-02-17.

* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]

* Dates converted to ISO 8601 format

* ISO DateTime with timezone (UTC) column added in ISO 8601 format.

* latitude and longitude columns added from values provided in submission metadata.

Data Files

File
948718_v1_epr_2019_nutrients.csv (Comma Separated Values (.csv), 1.26 KB) MD5:930a4d06384ec4f9b8d5122605527bd7 Primary data file for dataset ID 948718, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1756339

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AT42-09		2019-03-25	R/V Atlantis	Jason Brent Sylvan (Chief Scientist)	data info

Instruments

flatbed linear recorder

Supplied Name: PMC Industries Flat Bed Linear recorder

Supplied Description:

Technicon Bran & Luebbe AutoAnalyzer II (now owned by Seal Analytical) sampler, proportioning pump, manifold and colorimeter capable of analyzing for TDP as orthophosphate were used to measure phosphate concentration. A PMC Industries Flat Bed Linear recorder was used to record electronic output from the colorimeter.

Instrument Type

Generic Name: flatbed linear recorder

Generic Description:

flatbed linear recorder (chart recorder)
Example: PMC Industries Flat Bed Linear recorder which can be used to record electronic output from instruments.

Nutrient Autoanalyzer

Supplied Name: Seal AA500 System and AS2 sampler

Supplied Description:

To analyze colorimetry for nitrite an nitrite samples a Seal AA500 System and AS2 sampler, which includes proportioning pump, manifold and colorimeter capable of analyzing for nitrate plus nitrite was used.

Instrument Type

Generic Name: Nutrient Autoanalyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/

Generic Description:

Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples.

Nutrient Autoanalyzer

Supplied Name: Technicon Bran & Luebbe AutoAnalyzer II

Supplied Description:

Instrument Type

Generic Name: Nutrient Autoanalyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/

Generic Description:

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample_ID	Sample identifier provided by client, usually indicate on COC and/or sample vessel	unitless	sample
Depth	Depth of sample colection	meters (m)	depth
latitude	location sampled (latitude) Format: decimal degrees	decimal degrees	lat
longitude	location sampled (longitude)	decimal degrees	lon
Sample_Date	Date sample was taken or produced, usually indicated on COC and/or sample vessel. ISO 8601 format Format: %Y-%m-%d	unitless	date
Sample_Time	Time the sample was collected (recorded in UTC time zone). ISO 8601 format. Format: %H:%M:%S	unitless	time
Sample_ISO_DateTime_UTC	Datetime with timezone (UTC) sample was taken or produced, usually indicated on COC and/or sample vessel. ISO 8601 format Format: %Y-%m-%dT%H:%M:%SZ	unitless	ISO_DateTime_UTC
Received_Date	Date sample arrived at NASL/CBL and entered the analysis queue Format: %Y-%m-%d	unitless	date
NO2	Nitrite (NO2) concentration	milligrams per liter (mg N/L)	NO2
NO23	Nitrite (NO2) plus Nitrate (NO3) concentration	milligrams per liter (mg N/L)	NO3_NO2
PO4	Phosphate (PO4) concentration	milligrams per liter (mg P/L)	PO4
NO2_Flag	Flag for nitrite "NO2" column. Flag indicating if samples is below the method detection limit or reporting limit. An "L" denotes the sample concentration is less than the stated Method Detection Limit (MDL), BR denotes the concentration is less than the stated reporting limit. MDL is reported if the value is "L".	unitless	flag
NO23_Flag	Flag for nitrite plus nitrate column "NO23" indicating if samples is below the method detection limit or reporting limit. An "L" denotes the sample concentration is less than the stated Method Detection Limit (MDL), BR denotes the concentration is less than the stated reporting limit. MDL is reported if the value is "L".	unitless	flag
PO4_Flag	Flag for phosphate column 'PO4' indicating if samples is below the method detection limit or reporting limit. An "L" denotes the sample concentration is less than the stated Method Detection Limit (MDL), BR denotes the concentration is less than the stated reported limit. MDL is reported if the value is "L".	unitless	flag

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Dataset: EPR 2019 Nutrients