Dataset: Mussel respiration rates

Name: Mussel respiration data from experiments conducted at University of Washington Friday Harbor Laboratories, Friday Harbor, WA from 2020 to 2023
Published: 2025-02-18
Keywords: mussel, physiology, intertidal, thermal performance, oceans

View Data: Data not available yet

Cite This Dataset

Experiments were conducted at the University of Washington's Friday Harbor Labs on San Juan Island, WA, USA (48.545998N, 123.013046W).

Temporal Extent: 2021-06-14 - 2023-09-01

Project:

Collaborative Research: Microscale interactions of foundation species with their fluid environment: biological feedbacks alter ecological interactions of mussels (Microscale Mussels)

Principal Investigator:

Michael T. Nishizaki (Carleton College)

Student:

Katrin Birk (Carleton College)

Ruairi Brown (University of Washington Friday Harbor Laboratories, FHL)

Andrea Delgado (University of Washington Friday Harbor Laboratories, FHL)

Abdisalan Hawadle (University of Washington Friday Harbor Laboratories, FHL)

Ella Woodruff (Carleton College)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2025-02-18

Restricted:

Validated:

Current State:

Data not available

Mussel respiration data from experiments conducted at University of Washington Friday Harbor Laboratories, Friday Harbor, WA from 2020 to 2023

Abstract:

From 2021 to 2023, we measured aquatic respiration rates of three species of mytilid mussels (Mytilus trossulus Gould, M. galloprovincialis (L.), and M. californianus Conrad) under a range of water temperatures and velocities. M. californianus was obtained from the intertidal zone at Cattle Point, WA, USA (48.449966N, 122.964350 W) and shipped in chilled coolers to the University of Washington Friday Harbor Laboratories (48.545998N, 123.013046W). M. trossulus and M. galloprovinicialis were obtained from a commercial supplier (Penn Cove Shellfish, Coupeville, WA, USA). Mussels were acclimated in a recirculating seawater. We conducted lab experiments to investigate the effects of multple environmentl stressors (e.g., water temperature and velocity) on physiological responses in three closely-related mytilid mussel species. In closed respirometry chambers (15 cm × 5 cm × 5 cm, L × W × H), we measured oxygen use by individual mussels under a combination of five temperatures (e.g., 5, 11, 17, 23, and 29°C) and five velocities (2, 4, 6, 10, and 20 cm s-1) in a fully crossed design. Oxygen concentrations were measured with non-intrusive fiber-optic O2 sensors (Pyroscience, Aachen, DEU). Samples were recorded at a rate of 1 Hz and drift of the O2 probe was negligible (e.g., <0.1% over 2 h at 20°C). Trials were run for 2 h, ensuring that a stable rate of decline could be identified. After each trial, all soft tissues were dried for 72 hours at 60°C (Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA) and weighed using an analytical balance (0.001 g; PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA). Data were analyzed to estimate respiration rates using the R package respR (Harianto, 2019).

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Methods & Sampling

M. californianus Conrad, 1837 were obtained from Cattle Point on San Juan Island, WA (48°26'59.6811"N, 122°57'51.9358"W). M. trossulus and M. galloprovincialis were obtained from a commercial supplier (Penn Cove Shellfish, Coupeville, WA, USA; 48°13'06.5579" N, 122°42'25.4331"W). The mussels were maintained in seatables (66 × 135 × 32 cm) with a constant flow of seawater at the Friday Harbor Laboratories (48°32'45.7249" N, 123°00'46.9262"W). Mussels were fed twice daily with a total of approximately 10 mL of Shellfish Diet 1800 TM (Reed Mariculture, Campbell, CA, USA).

Aquatic respiration rates were measured in a closed, recirculating flow chamber of 1-liter capacity. The transparent acrylic test chamber (15 cm × 5 cm × 5 cm, L × W × H) was connected to a submersible pump (360 GPH, Model 25D, Rule Industries, Gloucester, MA, USA) via PVC pipe (25.4 mm i.d.) and custom machined high-density polyethylene connector fittings. The water-tight test chamber was filled with artificial seawater (32 PSU; Instant Ocean; Spectrum Brands, Blacksburg, VA) and submersed in a temperature-controlled water bath (50 L). The water bath's temperature was maintained using a programmable, recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA). Trials were run at water velocities were estimated along the centerline of the testing chamber by tracking the displacement of glass microbeads at each flow setting (mean particle diameter 9 μm, density 2.0 g cm⁻³; Potters Industries, Malvern, PA, USA).

Oxygen concentrations were measured with non-intrusive fiber-optic fluorescence-based optodes and sensor spots on the inner surface of the chamber (FireSting Pro; OXSP5 and TPSP5 spots, Pyroscience, Aachen, DEU). Samples were recorded at a rate of 1 Hz and drift of the O2 probe was negligible (e.g., <0.1% over 2 h at 20°C).

Each trial involved positioning individual mussels along the centerline of the chamber and subsequently measuring the organism's oxygen usage at stable temperatures of 5, 11, 17, 23, and 29°C. Trials were run for 2 h, ensuring that a stable rate of decline could be identified. After each trial, all soft tissues were dried for 72 hours at 60°C (Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA), and weighed using an analytical balance (0.001 g; PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA).

Problems/Issues

N/A

Data Processing Description

All analyses were conducted in R (ver. 4.4.2; RStudio 2024.12.0 (build 467). The R package respR was used to analyze the respiration data (Harianto, 2019). Respiration rates were standardized by the dry biomass of the mussels.

BCO-DMO Processing

* Data table within submitted file "Mytilus_edulis_hsp70.csv" was imported into the BCO-DMO data system for this dataset. Values "nd" imported as missing data values. Table will appear as Data File: 953794_v1_mussel-hsp70.csv (along with other download format options).

Missing Data Identifiers:
* In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.

* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]

* Date converted to ISO 8601 format

* LSIDs added for taxonomic names used in the metadata using the world register of marine species on 2025-02-18. Exact match to name used. "Mytilus edulis and M. californianus" edited to read as "Mytilus edulis and Mytilus californianus"

* Geospaital bounds added for organism source location in RI as indicated was the relevant location for this dataset. "Lab experiment at Carleton College in Northfild, MN conducted with organisms collected at Black Point in Narragansett Bay, RI "
41° 24′ 4.4964″ N, 71° 27′ 43.8228″ W converted to decimal degrees for entry: 41.401249,-71.462173

More Information about this dataset

Funding Sources