Dataset: pigments_CN
Deployment: LMG0602

POC, PON and Pigments from LMG0414 and LMG0602

Co-Chief Scientist:

Dr Laurence P. Madin (Woods Hole Oceanographic Institution, WHOI)

Principal Investigator:

Dr Patricia Kremer (University of Connecticut, UConn)

Project:

Salpa Thompsoni in the Southern Ocean: Bioenergetics, Population Dynamics and Biogeochemical Impact (SouthernSalps)

Current State:

Final no updates expected

Version:

4 March 2009

Version Date:

2009-03-04

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/452266/data

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Description

Particulate Carbon, Nitrogen and Pigments for LMG Cruises LMG0414 and LMG0602

Methods & Sampling

Dataset acquisition description

Sampling Protocol:
At each sampling station for salps, there was a CTD cast and water collected at several depths. Samples of water were filtered through both GF/F and GF/D (nominal 2um "pore") glass fiber filters. Filters to be used for elemental analysis (carbon and nitrogen) were pre-ashed (450 degC for 2-3 hours) and held in aluminum foil packets prior to use.

Data Processing Description

Dataset Processing Description

Analysis Protocol:
Pigment analysis of filters was done on shipboard. Immediately after water samples were filtered, filter pads were transferred into 6 ml of 90% acetone and the pigment was extracted in a dark refrigerator for 24 hours. Filter pads were removed and the test tubes centrifuged just prior to reading the fluorescence. Fluorescence was read with a Turner Designs 10 AU fluorometer. Following the initial fluorescence reading, samples were acidified with ~0.2ml of HCl and reread. The fluorometer was calibrated using a spectrophotometer during each cruise using standard Chl-a derived from spinach. Chlorophyll-a and total phaeopigments were calculated from standard equations (Strickland and Parsons 1972).

Immediately after filtration, pre-ashed filters for carbon and nitrogen were folded in half, placed in glassine envelopes, dried at 60degC in a drying oven, then stored in a -20deg C freezer and redried just prior to analysis. Elemental analysis was performed using a Carlo-Erba ES1500 elemental analyzer within 6 months of collection. Background values were determined using blanks from the same filter supply as the samples, but without water filtration.

More information about this dataset deployment

Funding

Award Number	Funding Source
ANT-0338290	NSF Antarctic Sciences

Instruments

CTD - profiler

Supplied Name: Conductivity, Temperature, Depth

Supplied Description:

Instrument Type

Generic Name: CTD - profiler

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/130/

Generic Description:

The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.

This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934.

Turner Designs Fluorometer 10-AU

Supplied Name: Turner Design Digital 10-AU-05 Fluorometer

Supplied Description:

Instrument Type

Generic Name: Turner Designs Fluorometer 10-AU

Acronym: Turner Fluorometer 10-AU

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/

Generic Description:

The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruiseid	Cruise identification. (e.g. LMG0414)	none	cruiseid
year	Year in GMT, YYYY, (e.g. 2004)	yyyy	year
date_gmt	month, day and year samples were collected	mm/dd/yyyy	date_gmt
lat	Latitude. North is positive.	decimal degrees	lat
lon	Longitude. South is negative.	decimal degrees	lon
operation	sample collection method, sometimes coupled with depth information	if depth present,meters	no_bcodmo_term
depth	Depth sample was collected.	meters	depth
filter_size	GFF = Glass Fiber Filters; 0.7um pore size.	microns	no_bcodmo_term
chla	Chlorophyll A; Plant pigment.	micrograms/kilogram	chl_a
phaeo	Phaeophytin, a Chlorophyll a degradation product resulting from the loss of the magnesium at the center of the chlorophyll a molecule.	micrograms/kilogram	phaeo
total_pig	chlorophyll a + phaeo	micrograms/kilogram	no_bcodmo_term
pcnt_phaeo	Percent phaeophytin in the sample	percent	no_bcodmo_term
pcnt_chl_tot	percent chlorophyll of the total pigment in the sample	percent	no_bcodmo_term
N	elemental Nitrogen	micrograms per liter	no_bcodmo_term
C	elemental Carbon	micrograms per liter	no_bcodmo_term
C_Chl_rat	ratio of Carbon to Chlorophyll in the sample	unitless	no_bcodmo_term
C_N_rat	ratio of Carbon to Nitrogen in the sample	unitless	no_bcodmo_term

Database

Contribute Data

Dataset: pigments_CN
Deployment: LMG0602

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: pigments_CNDeployment: LMG0602

Dataset acquisition description

Dataset Processing Description

Dataset: pigments_CN
Deployment: LMG0602