Dataset: Discrete - 13C samples
Deployment: KM0812

See Platform Deployments for cruise specific documentation

# Discrete samples of 13C fixation rates
# CMORE Affiliated /POOB
# Ocean Microbial Ecology Laboratory
# Ricardo Letelier
#
# original file: White_15N13C.xls
# originally ingested into BCO-DMO: December 6, 2010
# updated: Feb 11, 2011 (added ancillary columns - data unchanged)
#
A total of 0.5 ml of 47 mM bicarbonate (NaH¹³CO₃) stock was injected into a 4L polycarbonate bottle using a Leur-lock syringe and the bottles were gently mixed by inversion. Samples were incubated at appropriate light levels and temperature for 24 hours. Duplicate samples were taken when adequate water was available. Incubations were terminated by filtering the entire incubation volume onto a 25 mm pre-combusted glass fiber filter (GF/F, Whatman); following filtration, the filters were stored at -20°C until later analysis. Once ashore, samples were acid-fumed, dried overnight at 60°C and then encapsulated in tin and silver capsules. Particulate C, N and the isotopic composition of particulate material (δ¹⁵N_PN and δ¹³C_PC) were determined using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer in the University of California Davis stable isotope facility.