Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Methods described in Johnson et al. 2010: Chlorophyll concentrations were measured by filtering 20 mL of seawater sample onto a 0.22 micrometer (um) pore size polycarbonate filter using gentle vacuum (<100 mm Hg) and extracting in 100% MeOH at -20 degrees C in the dark for >24 h following Holm-Hansen and Riemann (1978). Fluorescence was measured using a Turner Designs 10-AU fluorometer following Welschmeyer (1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2006).
References:
Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. 2010. The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: 10.1357/002224010793721433
Holm-Hansen, O., and Riemann, B. 1978. Chlorophyll a determination: Improvements in methodology. Oikos 30: 438-447.
Welschmeyer, N.A. 1994. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography 39: 1985-1992.
Ritchie, R. 2008. Universal chlorophyll equations for estimating chlorophylls a, b, c , and d and total chlorophylls in natural assemblages of photosynthetic organisms using acetone, methanol, or ethanol solvents. Photosynthetica 46: 115-126. doi: 10.1007/s11099-008-0019-7
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