Dataset: Phytoplankton - Microphytoplankton Underway Counts
Deployment: AT21-04

Phytoplankton - Microphytoplankton Underway Counts

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/473815

Principal Investigator:

Edward J. Carpenter (San Francisco State University, SFSU)

Student:

Mr Andrew Kalmbach (San Francisco State University, SFSU)

Contact:

Edward J. Carpenter (San Francisco State University, SFSU)

BCO-DMO Data Manager:

Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Amazon iNfluence on the Atlantic: CarbOn export from Nitrogen fixation by DiAtom Symbioses (ANACONDAS)

Current State:

Preliminary and in progress

Version:

02 January 2014

Deployment Synonyms:

AN12, ANACONDAS, ROCA

Version Date:

2014-01-02

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Description

Phytoplankton species counts taken at hourly intervals from the ships seawater system while underway
Phytoplankton species composition and cells per ml

Methods & Sampling

Dataset acquisition description

When the ship was underway between stations, from 2 to 6 Liters (depending on cell density) were collected from the ship’s seawater system and water was filtered through a 20 micrometer Nitex mesh filter. Collection was done on an hourly basis through the entire day and night as long as the ship was moving. Cells were then backwashed off the netting with filtered seawater into a 50 ml Falcon tube. These cells were preserved with acid Lugols solution. On shore, 1 ml of the concentrate was placed in a Sedgwick Rafter counting chamber and species were identified and enumerated at 200X in transects in the chamber using a Zeiss Axioskop microscope.

Data Processing Description

Dataset Processing Description

(See Processing Notes for Individual Deployments)

Deployment-specific processing description

Notes from original spreadsheet for: AT21-04
These are the phytoplankton counts from the Atlantis in July 2012, we sampled at each station and underway
Underway counts were collected from the ships uncontaminated seawater system, with the intake located 2.6 m below the surface.
All samples were filtered through 20 um mesh to concentrate down to about 50 ml, and fixed with Acid lugol's solution
Most samples were further concentrated in a centerfuge for 10 minutes at 2000 RPM (15 ml down to 1 ml)
Concentrate was placed in a Sedgewick Rafter counting chamber, and counted under 320x magnification

Counts in Cells per liter

Collected by ANACONDAS (See UW Records sheet)
Counted by EJC, AJK
Data Entry by HLR, AJK
Data corrrection by MTM

BCO-DMO Processing Notes
- Generated from original file: "At21-04 Microphytoplankton underway counts.xlsx" contributed by Andrew Kalmbach
- Cruise "Id_Year" added to data (i.e. "AN12_2012")
- Event Date/Time GMT added from event logs
- Event Lat/lon added from event logs
- Parameters modified to conform to BCO-DMO parameter naming conventions
- "nd" (Not Detected) inserted into blank cells

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0934035	NSF Division of Ocean Sciences

Instruments

Pump - Surface Underway Ship Intake

Supplied Name: pump-ship intake

Supplied Description:

Instrument Type

Generic Name: Pump - Surface Underway Ship Intake

Acronym: Pump-Ship Intake

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/31/

Generic Description:

The 'Pump-underway ship intake' system indicates that samples are from the ship's clean water intake pump. This is essentially a surface water sample from a source of uncontaminated near-surface (commonly 3 to 7 m) seawater that can be pumped continuously to shipboard laboratories on research vessels. There is typically a temperature sensor near the intake (known as the hull temperature) to provide measurements that are as close as possible to the ambient water temperature. The flow from the supply is typically directed through continuously logged sensors such as a thermosalinograph and a fluorometer. Water samples are often collected from the underway supply that may also be referred to as the non-toxic supply. Ideally the data contributor has specified the depth in the ship's hull at which the pump is mounted.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Id_Year	ANACONDAS Cruise Id and Year of Cruise (ANYY_YYYY)	text	cruise_id
AN_Number	ANACONDAS Sample Id (Sample Id = CruiseIdxxxxx)	text	sample
Station	ANACONDAS Station Id	text	station
Event_Number	ANACONDAS Event Id (Event Id = Station Id.Event Id at station)	text	event
Date_GMT	Event Date (GMT)	YYYYMMDD	date
Time_GMT	Event Time (GMT)	HHMM	time
Latitude	Event Latitude (South is negative)	decimal degrees	lat
Longitude	Event Longitude (West is negative)	decimal degrees	lon

Database

Contribute Data

Dataset: Phytoplankton - Microphytoplankton Underway Counts
Deployment: AT21-04

Dataset acquisition description

Dataset Processing Description

Deployment-specific processing description

Database

Contribute Data

Dataset: Phytoplankton - Microphytoplankton Underway CountsDeployment: AT21-04

Dataset acquisition description

Dataset Processing Description

Deployment-specific processing description

Dataset: Phytoplankton - Microphytoplankton Underway Counts
Deployment: AT21-04