Dataset: TN-BS water inhibition

Name: Inhibition data for 3 peptidases at Bogue Sound NC and Tennessee River at Knoxville (SEDpep project)
Published: 2016-03-14
Keywords: oceans

Cite This Dataset

Project:

Novel peptidases in subsurface sediments: Activities and substrate specificities (SEDpep)

Program:

Center for Dark Energy Biosphere Investigations (C-DEBI)

Principal Investigator:

Andrew Steen (University of Tennessee Knoxville, UTK)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

14 March 2016

Version Date:

2016-03-14

Restricted:

Validated:

Current State:

Preliminary and in progress

Inhibition data for 3 peptidases at Bogue Sound NC and Tennessee River at Knoxville (SEDpep project)

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Description

Inhibition data for 3 peptidases at Bogue Sound NC and Tennessee River at Knoxville.

Methods & Sampling

Inhibition parameters were measured from vo of Arg-AMC, Leu-AMC, and Pro-AMC, as a function of the concentration of various pNA inhibitors, as described in Steen et al (2015) (doi:10.3354/ame01755)

Data Processing Description

Data processing is described in more detail in the Steen et al. (2015) paper (doi:10.3354/ame01755). Full R code and all raw data is given at http://github.com/adsteen/subspec.

BCO-DMO processing:
- modified parameter names to conform with BCO-DMO naming conventions;
- replaced "NA" with "nd" (no data).

Data Files

File
TN-BS_water_inhibition.csv (Comma Separated Values (.csv), 13.64 KB) MD5:d3e0af3502c061f1f5e4625f47bf5bf7 Primary data file for dataset ID 640505

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0939564

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
amino_acid	Amino acid.	dimensionless	no_bcodmo_term
AA_abbrev	Single-letter amino acid abbreviation.	dimensionless	no_bcodmo_term
delta_Gr	?	?	no_bcodmo_term
pNA_subs	?	?	no_bcodmo_term
polarity	Description of polarity.	dimensionless	no_bcodmo_term
hydrophobicity	Hydrophobicity.	?	no_bcodmo_term
MW	Molecular weight of the amino acid.	grams per mole	no_bcodmo_term
Dauwe_score1	?	?	no_bcodmo_term
Dauwe_score2	?	?	no_bcodmo_term
location	Sampling location.	dimensionless	no_bcodmo_term
substrate	Substrate.	dimensionless	no_bcodmo_term
inhib_slope	Inhibition slope.	?	no_bcodmo_term
inhib_slope_SE	Standard error of inhibition slope.	?	no_bcodmo_term
rsq	?	?	no_bcodmo_term
pval	?	?	no_bcodmo_term
is_homologous	?	dimensionless	no_bcodmo_term
rel_Ki	Inhibition binding constant, Ki. Given relative to the homologous pNA substrate, as described in Steen et al. (2015).	?	no_bcodmo_term
rel_Ki_SE	Standard error of inhibition binding constant, Ki. Given relative to the homologous pNA substrate, as described in Steen et al. (2015).	?	no_bcodmo_term
low_err	?	?	no_bcodmo_term
high_err	?	?	no_bcodmo_term

Status message

Database

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Dataset: TN-BS water inhibition