Dataset: Grazing experiment 7: cell size
Deployment: Lab_Olson_B

Cell volume measurements of low-high pCO2 acclimated Rhodomonas

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/670211/data

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Description

Related Reference:
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO₂ induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

Dataset acquisition description

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. On days 10 and 16, Rhodomonas cells from the treatments were mounted live on a microscope slide and 50 cells from each treatment replicate were imaged using RSImage software under 400X magnification on an Olympus CHA microscope. ImageJ software was used to measure Rhodomonas length and width. Rhodomonas cells are described as having a prolate spheroid shape. The volume was calculated using: Vprolate(µm3) =(4/3)πa²b. Where a=1/2 width and b=1/2 length of the Rhodomonas cell.

Data Processing Description

Dataset Processing Description

These data are unprocessed cell sizes as calculated above.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- combined day 10 and day 16 data
- added day column

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961229	NSF Division of Ocean Sciences

Instruments

Microscope - Optical

Supplied Name: Olympus CHA microscope

Supplied Description:

Used to measure Rhodomonas cells

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
day	experiment day	day	day
expt_day_treatment_rep_cell_number	sample identifier: individual cell measured: experiment day_pCO2 level_replicate_cell number	unitless	sample
length	cell length	micometers	length
width	cell width	micometers	width
volume	cell volume	micometers	volume

Database

Contribute Data

Dataset: Grazing experiment 7: cell size
Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Grazing experiment 7: cell sizeDeployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Dataset: Grazing experiment 7: cell size
Deployment: Lab_Olson_B