Dataset: AR16 Experiment Chl a
Deployment: AR16

Chlorophyll a concentrations from addition incubation experiments performed on AR16

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/775742

Principal Investigator:

Tatiana A. Rynearson (University of Rhode Island, URI-GSO)

Co-Principal Investigator:

Sonya T. Dyhrman (Columbia University)

Bethany D. Jenkins (University of Rhode Island, URI-GSO)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic (North Atlantic Diatoms)

Current State:

Preliminary and in progress

Version:

Deployment Synonyms:

PHAT – Phosphorus, Hydrocarbons, and Trichodesmium

Version Date:

2019-08-21

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Description

Chlorophyll a concentrations from addition incubation experiments performed on AR16.

Methods & Sampling

Dataset acquisition description

In situ Chlorophyll a was measured at the conclusion of each incubation experiment conducted in triplicate on AR16. Samples were taken from biological replicates and filtered onto GF/F and 5µm polycarbonate filters.

Chl a was extracted from filters in 100% ethanol for 12 hours, measured on a 10-AU Fluorometer, and chlorophyll a and phaeophytin concentrations were calculated based on a predetermined calibration curve. Concentrations on each filter were used to determine total chl a within each size fraction.

Data Processing Description

Dataset Processing Description

Data was processed in R 3.4.1 (R-Core-Team 2017).

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- removed greater than symbol (>) from size_fraction field.
- reformatted date from mm/dd/yy HH:MM to yyyy-mm-ddTHH:MM:SS to conform with the ISO 8601 convention

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1558490	NSF Division of Ocean Sciences

Instruments

Turner Designs Fluorometer 10-AU

Supplied Name: 10AU Fluorometer

Supplied Description:

Instrument Type

Generic Name: Turner Designs Fluorometer 10-AU

Acronym: Turner Fluorometer 10-AU

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/

Generic Description:

The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
datetime	Date and time of incubation conclusion in UTC following ISO 8601 format	unitless	ISO_DateTime_UTC
experiment	Experiment number in series	unitless	exp_id
treatment	Experimental treatment	unitless	treatment
bottle_number	Incubation bottle that was sampled	unitless	bottle
replicate	Biological replicate of triplicate incubations	unitless	replicate
size_fraction	greater than size fraction analyzed based on pore size of filter	micrometers (um)	no_bcodmo_term
vol_filtered	Volume of seawater filtered for chl a analysis	milliliters (ml)	vol_filt
vol_extracted	Volume of ethanol used to extract chla a	milliliters (ml)	volume
Fb	Fluorescence before acidification	RFU	fluor
Fa	Fluroescence after acidification	RFU	fluor
Notes	Notes regarding sample	unitless	comment
blank_subt_Fb	Blank corrected fluorescence before acidification	RFU	fluor
blank_subt_Fa	Blank corrected fluorescence after acidification	RFU	fluor
chla	Chlorophyll a concentration	micrograms per liter (ug/L)	chl_a
phaeo	Phaeophytin concentration	micrograms per liter (ug/L)	phaeo

Database

Contribute Data

Dataset: AR16 Experiment Chl a
Deployment: AR16

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: AR16 Experiment Chl aDeployment: AR16

Dataset acquisition description

Dataset Processing Description

Dataset: AR16 Experiment Chl a
Deployment: AR16