Dataset: Retinal Response

Name: Retinal responses during exposure to decreasing oxygen partial pressure (pO2)
Published: 2019-10-23
Keywords: electrophysiology, hypoxia, marine invertebrate, oxygen, vision, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.779540.1

Project:

Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae (Vision under hypoxia)

Principal Investigator:

Lisa A. Levin (University of California-San Diego, UCSD)

Co-Principal Investigator:

Nicholas Oesch (University of California-San Diego, UCSD)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-10-23

Restricted:

Validated:

Current State:

Preliminary and in progress

Retinal responses during exposure to decreasing oxygen partial pressure (pO2)

Abstract:

Retinal responses during exposure to decreasing oxygen partial pressure (pO2) in marine invertebrate larvae determined with electroretinograms.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2019-10-23	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.779540.1	2019-11-01

Description

Retinal responses during exposure to decreasing oxygen partial pressure (pO2) in marine invertebrate larvae determined with electroretinograms.

Please see additional datasets for this paper, including the “OxygenMetrics_Vision” and the “Experiment_Metrics” datasets.

Methods & Sampling

Detailed methods can be found in McCormick et al., 2019

Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m−2 s−1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution.

This dataset shows the retinal responses measured every 20 s for all individuals used in Figure 1 (top panel) of McCormick et al., 2019. The retinal responses show a decline in retinal function during a decrease in oxygen availability.

Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.

Data Processing Description

All electrophysiology data was recorded and analyzed using Igor Pro 7 Software (Wavemetrics) using custom code. Oxygen data was analyzed using PreSens Measurement Studio 2. Post-processing analysis was completed in R Studio (version 3.3.3).

Data Files

File
retinal_response.csv (Comma Separated Values (.csv), 185.63 KB) MD5:2190f8807d89dc2c900d031f7a6b4967 Primary data file for dataset ID 779540

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1829623

Deployments

None available yet

Instruments

Oxygen Microelectrode Sensor

Supplied Name:

Supplied Description:

Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.

Instrument Type

Generic Name: Oxygen Microelectrode Sensor

Acronym: O2 Microelectrode

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/351/

Generic Description:

Any microelectrode sensor that measures oxygen.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Species	Species name. Format is "Species_genus".	unitless	species
Experiment_Name	Original experiment name that can be linked back to raw datafile collected in Igor.	unitless	exp_id
Round	The number of seconds since starting each individual experiment.	unitless	time_elapsed
O2_umol_l	Oxygen concentration in umol O2/L for each retinal measurement.	micromole per liter (umol/L)	O2
O2_ml_l	Oxygen concentration in mL O2/L for each retinal measurement.	milliliter per liter (ml/l)	O2
O2_umol_kg	Oxygen concentration in umol O2/kg for each retinal measurement.	micromole per kg (umol/kg)	O2
O2_pO2	The partial pressure of oxygen of the given metric “n.ctrl.area”: The ERG response normalized to the average retinal response measured during exposure to normoxia.	kiloPascal (kPa)	no_bcodmo_term
n_ctrl_area	The integration of the ERG wave at any given time divided by the average integrated ERG during control oxygen conditions. Normalization was done to compare between individuals and species.	unitless	no_bcodmo_term
RunningAvg	The running average of retinal responses in the last minute, used to show smoothed responses over time.	unitless	no_bcodmo_term
RunAvgLine	The line number for the running average, used to order measurements within an individual experiment.	unitless	no_bcodmo_term
Condition	Oxygen exposure during the experiment, either "normoxia", “decrease”, or “Low”. First, animals were exposed to “normoxia” (~100-105% oxygen saturation), then a “Deacrease” in oxygen (slow decline of oxygen), until the “Low” oxygen was reached.	unitless	no_bcodmo_term

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