Dataset: Sample Metadata for MOCNESS Tows
Deployment: KM1910

Sample Metadata for MOCNESS Tows

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/854424

Principal Investigator:

Erica Goetze (University of Hawaiʻi at Mānoa, SOEST)

Co-Principal Investigator:

Matthew J. Church (University of Montana)

Sara Ferrón (University of Hawaiʻi at Mānoa, SOEST)

Angelicque E. White (University of Hawaiʻi at Mānoa, SOEST)

Contact:

Erica Goetze (University of Hawaiʻi at Mānoa, SOEST)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

EAGER Collaborative Research: Early career chief scientist training for biological and chemical oceanographers (Chief Sci KM1910)

Version:

Deployment Synonyms:

EAGER, Chief Sci cruise, 2019 Chief Scientist Training Cruise

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Description

Data have been published “as is”. Final review by the data submitter was not received after it was imported into the BCO-DMO data system.

Methods & Sampling

Dataset acquisition description

The zooplankton assemblage was collected using a 1m2 Multiple Opening/Closing Nets and Environmental Sampling System (MOCNESS), with all nets and cod ends at 202 µm mesh. All tows were conducted at Station ALOHA (22° 45’N 158° W) A total of 5 tows sampled the full water column between 1000 m and the sea surface, with 3 day and 2 night paired tows. Depth strata were as follows: 0-1000m (Net 0), 1000-800 m (Net 1), 800-600m (Net 2), 600-500m (Net 3), 500-400m (Net 4), 400-300m (Net 5), 300-200m (Net 6), 200-100m (Net 7), 100-50m (Net 8), 50-0m (Net 9).

Bulk plankton from each net and tow were quantitatively split using a Folsom plankton splitter, with additional processing or preservation for several analyses. Two additional ‘short’ MOCNESS tows were conducted to sample the base of the mesopelagic (700-1000m) and epipelagic (0-200 m) for metatranscriptomic analyses of the zooplankton assemblage. Rapid recovery and preservation of the material was important in this case, given primary interest in gene expression profiles, and so the nets were flown as quickly as possible through midwater.

Quantitative subsamples of bulk plankton were preserved as follows:

Formalin-preserved – sodium tetraborate buffered formaldehyde, preserved broadly following CalCOFI protocols (https://calcofi.org/about-calcofi/calcofi-handbook/301-net-sample-preservation.html). Analyses: ZooScan, Microscopy
Size-fractionated into 5 or 2 fractions (0.2-0.5 mm, 0.5-1.0 mm, 1.0-2.0 mm, 2.0-5.0 mm, >5.0 mm OR 0.2-1.0 mm, >1.0 mm), filtered onto 200 µm Nitex filters, rinsed with ammonium formate, and followed by cryopreservation and storage at -80° C. Analyses: biomass, bioelements, isotopes.
Filtered onto 200 µm Nitex filters, followed by preservation in RNALater and storage at -80° C. Analyses: Metabarcoding.
Filtered onto 200 µm Nitex filters, flash frozen and stored in liquid nitrogen. Analyses: Electron Transport System (ETS) activity assay.
Size-fractionated into 2 fractions (0.2 - 1.0 mm, > 1.0 mm) and analyzed shipboard for alkaline phosphatase activity (APA assay)

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:

renamed fields to remove spaces, and problematic characters
converted time to UTC
converted lat lon to decimal degrees
split size fraction column into min and max values
split target depths column into min and max values

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1911831	NSF Division of Ocean Sciences
OCE-1911990	NSF Division of Ocean Sciences

Instruments

Folsom Plankton Splitter

Supplied Description:

Bulk plankton from each net and tow were quantitatively split using a Folsom plankton splitter, with additional processing or preservation for several analyses.

Instrument Type

Generic Name: Folsom Plankton Splitter

Acronym: Folsom Splitter

Generic Description:

A Folsom Plankton Splitter is used for sub-sampling of plankton and ichthyoplankton samples.

MOCNESS1

Supplied Name: 1m2 MOCNESS

Supplied Description:

The zooplankton assemblage was collected using a 1m2 Multiple Opening/Closing Nets and Environmental Sampling System (MOCNESS), with all nets and cod ends at 202 µm mesh. All tows were conducted at Station ALOHA (22° 45’N 158° W). A total of 5 tows sampled the full water column between 1000 m and the sea surface, with 3 day and 2 night paired tows. Depth strata were as follows: 0-1000m (Net 0), 1000-800 m (Net 1), 800-600m (Net 2), 600-500m (Net 3), 500-400m (Net 4), 400-300m (Net 5), 300-200m (Net 6), 200-100m (Net 7), 100-50m (Net 8), 50-0m (Net 9).

Instrument Type

Generic Name: MOCNESS1

Acronym: MOC1

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/NETT0097/

Generic Description:

The Multiple Opening/Closing Net and Environmental Sensing System or MOCNESS is a family of net systems based on the Tucker Trawl principle. The MOCNESS-1 carries nine 1-m2 nets usually of 335 micrometer mesh and is intended for use with the macrozooplankton. All nets are black to reduce contrast with the background. A motor/toggle release assembly is mounted on the top portion of the frame and stainless steel cables with swaged fittings are used to attach the net bar to the toggle release. A stepping motor in a pressure compensated case filled with oil turns the escapement crankshaft of the toggle release which sequentially releases the nets to an open then closed position on command from the surface. -- from the MOCNESS Operations Manual (1999 + 2003).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
ID	Unique record idenfitier	unitless	sample
Event_no	Event identifier	unitless	event
Tow_no	Tow identifier	unitless	tow
Latitude_decimal_degrees	Latitude (degrees N)	decimal degrees	lat
Longitude_decimal_degrees	Longitude (degrees E)	decimal degrees	lon
Time_HST	Local time	unitless	time_local
Time_UTC	Time in UTC	unitless	time_utc
Day_vs_night_tow	Time of day when a zooplankton sample was taken. "N" indicates night, and "D" indicates a daytime sample. "N short" and "D short" correspond to 2 abbreviated MOCNESS tows conducted to sample zooplankton for metatranscriptomics in the epipelagic and deep mesopelagic.	unitless	time_of_day
Net_no	Net identifier	unitless	net
Target_depths_m	Target collection depth range	meters (m)	depth
Target_depth_min_m	Target depth minimum	meters (m)	depth_min
Target_depth_max_m	Target depth maximum	meters (m)	depth_max
Bulk_fraction	Bulk fraction is the quantitative subsample of the original bulk plankton from that tow/net.	unitless	sample_descrip
Size_fraction	Size fractions as follows: NSF = not size-fractionated, 0.2-1.0mm [200 um - 1000 um], > 1.0mm [ > 1000 um].	milimeters (mm)	sample_descrip
Size_fraction_min	Size fraction minimums as follows: NSF = not size-fractionated, 0.2-1.0mm [200 um - 1000 um], > 1.0mm [ > 1000 um].	milimeters (mm)	sample_descrip
Size_fraction_max	Size fractions maximum as follows: NSF = not size-fractionated, 0.2-1.0mm [200 um - 1000 um], > 1.0mm [ > 1000 um].	milimeters (mm)	sample_descrip
Purpose_analysis	Purpose of collection and subsiquent analysis	unitless	comment
Preservation	Preservation method applied to collected plankton	unitless	comment

Database

Contribute Data

Dataset: Sample Metadata for MOCNESS Tows
Deployment: KM1910

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Sample Metadata for MOCNESS TowsDeployment: KM1910

Dataset acquisition description

Dataset Processing Description

Dataset: Sample Metadata for MOCNESS Tows
Deployment: KM1910