Dataset: BF Bioassay Experiments
Deployment: SAV-19-02

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/864157

Principal Investigator:

Solange Duhamel (Columbia University)

Co-Principal Investigator:

Julia Diaz (University of Georgia, UGA)

Contact:

Kahina Djaoudi (University of Arizona, UA)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)

Version:

Deployment Synonyms:

Zephyr, Zooming in on Enzymatic PhosphoHYdrolysis Reactions

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Description

Methods & Sampling

Dataset acquisition description

Sampling and analytical procedures:

Bioassay experiments were conducted at station 1 and stations 3. At each station, inorganic and organic phosphate amendments were performed on seawater with and without nitrogen enrichment (NH₄Cl, NaNO₃). Bioassay Experiments consisted in incubating, over an incubation period of 48h, surface seawater (5m) with inorganic or organic phosphate compounds (20 µM; final concentration of P) including, polyphosphate (polyp), inorganic phosphate (Pi), nucleotides (ATP or AMP) and methylphosphonate (Mepn). In each incubation experiment, a control treatment (surface seawater without amendment) was included.

In bioassay experiments, the bioavailability factor (BF) was determined at T0 for polyphosphate, nucleotides (ATP or AMP) and methylphosphonate (Mepn). BF is calculated as follows: BF=TE-TN/ TP-TN (Björkman and Karl, 1994), where T_E reflects PO₄^3- turnover time in the DOP amended treatment, T_N is the PO₄^3-turnover time in the control (no additions) and T_P is the PO₄^3-turnover time in the treatment amended with Pi. BF ranges from 0 for an unavailable substrate, to a value of 1 for a DOP model substrate having a bioavailability equal to that of +Pi.

Instrument: Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.
Location: Northwestern Atlantic surface waters. Depth: surface-50 m.

Data Processing Description

Dataset Processing Description

Data were organized using MATLAB and output as .mat files. Gaps in data were filled with NaN in the .mat files.

BCO-DMO Data Manager Processing Notes:

* Data from source files BioassayExperiments_Datasets.xslx. Sheets: BF_T0_St1, BF_T0_St1_NN, BF_T0_St3 were imported into the BCO-DMO data system and combined into one data table.
* Data columns were added to contain information that was in the Excel sheet names. The original excel sheet name was added as column data_subset_name. Information extracted to additional columns (incubation_hours, station)
* Parameters (column names) renamed to comply with BCO-DMO naming conventions. See https://www.bco-dmo.org/page/bco-dmo-data-processing-conventions
* Missing data values (Blank/Null) values in this dataset are displayed according to the format of data you access. For example, in csv files it will be blank values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as "nd" meaning "no data."

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1736967	NSF Division of Ocean Sciences
OCE-1737083	NSF Division of Ocean Sciences
OCE-1948042	NSF Division of Ocean Sciences
OCE-2001212	NSF Division of Ocean Sciences

Instruments

Liquid Scintillation Counter

Supplied Name: Packard Tri-Carb liquid scintillation counter

Supplied Description:

Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.

Instrument Type

Generic Name: Liquid Scintillation Counter

Acronym: LSC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Generic Description:

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
data_subset_name	Data subset name	unitless	brief_desc
Id_treatments	Labels of dissolved inorganic/organic phosphate amended treatments. Treatments that were amended with Nitrogen (NH4Cl, NaNO3) to avoid nitrogen limitation contain "+NN" in the treatment ID. Acronyms (e.g. Mepn, AMD) are explained in the "Sampling and analytical procedures" section.	unitless	treatment
station	Station at which the treatments were conducted	unitless	station
incubation_hours	Incubation hours. (T=0 is the start of the treatment).	hours	time_elapsed
BF	Bioavailability factor (0-1). See methodology for calculations.	unitless	no_bcodmo_term

Database

Contribute Data

Dataset: BF Bioassay Experiments
Deployment: SAV-19-02

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: BF Bioassay ExperimentsDeployment: SAV-19-02

Dataset acquisition description

Dataset Processing Description

Dataset: BF Bioassay Experiments
Deployment: SAV-19-02