Dataset: Blank sources

Name: Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted from 2017-2020 (Biological Nitrogen Isotope Fractionation project)
Published: 2021-11-16
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.865089.1

Temporal Extent: 2017-08-01 - 2020-06-08

Project:

CAREER: The biological nitrogen isotope systematics of ammonium consumption and production (Biological Nitrogen Isotope Fractionation)

Principal Investigator:

Julie Granger (University of Connecticut, UConn)

Student:

Mengyang Zhou (University of Connecticut, UConn)

Contact:

Mengyang Zhou (University of Connecticut, UConn)

Analyst:

Bonnie X. Chang (University of Washington, UW)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-16

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted from 2017-2020 (Biological Nitrogen Isotope Fractionation project)

Abstract:

Volume-dependent N2O blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2021-11-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.865089.1	2022-01-14

Methods & Sampling

Methodology:

Sampling and analytical procedures:

 Effects of sample volume on the size of bacterial blanks

Following sparging with N2 gas, bacterial concentrates (2 mL) in 20 mL vials were injected with incremental volumes of DIW or NO3--deplete surface Sargasso seawater. Solutions included air-equilibrated DIW (for ≥ 1 day) and helium-sparged DIW (for 30 minutes), as well as air-equilibrated vs. helium-sparged seawater. N2O yields were estimated from peak areas recovered by mass spectrometric analysis, calibrated with standard additions.

Data Processing Description

Processing notes from submitting researcher:

Data were processed in Microsoft Excel

BCO-DMO processing notes

Date formats were changed from mm/dd/yy to yyyy-mm-dd
Spaces and units removed from column headers

Data Files

File
zhou_et_al_lab_data-5.csv (Comma Separated Values (.csv), 12.56 KB) MD5:0111ffe3b3f0b354c3cbf07c03320a48 Primary data file for dataset ID 865089

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1554474

Deployments

None available yet

Instruments

Gas Chromatograph Mass Spectrometer

Supplied Name: isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)

Supplied Description:

Delta V Advantage continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) interfaced with a modified Thermo Fisher Scientific Gas Bench sample preparation device fronted by dual cold traps (Casciotti et al., 2002) and a GC Pal autosampler (CTC Analytics, Zwingen, Switzerland) - to measure N and O isotope ratio of nitrate using the denitrified method.

Instrument Type

Generic Name: Gas Chromatograph Mass Spectrometer

Generic Description:

Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Strain	The two denitrifying bacteria strains used in the laboratory experiment: P. aureofaciens and P. chlororaphis	unitless	species
Date	Date of the experiments; yyyy-mm-dd Format: %Y-%m-%d	unitless	date
Trial	Trial name	unitless	replicate
Solution	Type of aliquot. DIW or nitrate-deplete surface Sargasso seawater	unitless	sample_type
Sample_processiong	Solutions were equilibrated in the air for ≥ 1 day, or sparged with helium or N2 gas for 30 minutes	unitless	sample_type
Sample_volume	Volume of solution addition	mL	volume
Blank_N2O	The amount of blank N2O recovered with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler	nmol of N	N2O

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Database

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Dataset: Blank sources