Dataset: Shell thickness of mussel recruits

Name: Shell thickness of mussel recruits quantified in two species, Mytilus trossulus and Mytilus californianus
Published: 2024-01-24
Keywords: Mytilus californianus, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.918420.1

Spatial Extent: N:44.244 E:-121.929 S:36.4476 W:-124.114

Bodega Marine Reserve and Soberanes Point, California; and Bob Creek, Oregon

Temporal Extent: 2020-06-12 - 2021-03-11

Project:

Coastal mosaics of local adaptation and the eco-evolutionary dynamics of a marine predator-prey interaction (Coastal Adaptation)

Principal Investigator:

Eric Sanford (University of California-Davis, UC Davis-BML)

Student:

Emily K. Longman (University of California-Davis, UC Davis-BML)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-01-24

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Shell thickness of mussel recruits quantified in two species, Mytilus trossulus and Mytilus californianus

Abstract:

Mussels are common on rocky shores along the west coast of North America and are prey to a number of species, including the Channeled Dogwhelk, Nucella canaliculata. When N. canaliculata hatch, they are dependent on a supply of newly recruited prey that are variable in abundance. To determine the strength of selection that early-life diet may impose on juvenile dogwhelks, recruit mussel shell thickness was quantified for two mussel species, Mytilus trossulus and Mytilus californianus. In addition, recruit M. californianus were collected from two locations, Bodega Marine Reserve and Soberanes Point, known to differ in the thickness of adult M. californianus.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2024-01-24	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.918420.1	2024-01-26

Methods & Sampling

Recruit Mytilus trossulus mussels were collected from Bob Creek, Oregon. Recruit M. californianus were collected from Bodega Marine Reserve, California and Soberanes Point, California. Recruit mussel shell thickness comparisons were made for approximately 55 mussels per mussel type across the range of mussel sizes given to juvenile N. canaliculata. Mussel tissue was removed by incubating the mussels in 15% H2O2, 0.05 M NaOH with period agitation (Gaylord et al. 2018). The mussel shells were then dried to a constant weight and dry weights were measured on a microbalance. Pictures of the shells were taken using a camera (Leica MC170) attached to a dissecting microscope (Leica M125) and were used to calculate shell area and mussel length. These pictures are in the attached Supplemental File "Mussel Recruit Images.zip". Recruit mussel shell thickness was estimated as the total dry weight of both valves divided by the total projected area of both valves (Gaylord et al. 2018).

BCO-DMO Processing

- Imported original file "Shell thickness of mussel recruits.xlsx" into the BCO-DMO system.
- Flagged "NA" as a missing data value (missing data are empty/blank in the final CSV file).
- Added columns for site Latitude and Longitude.
- Renamed fields/columns to comply with BCO-DMO naming conventions.
- Replaced commas with semi-colons in the "Notes" column.
- Saved the final file as "918420_v1_shell_thickness_of_mussel_recruits.csv"

Data Files

File
918420_v1_shell_thickness_of_mussel_recruits.csv (Comma Separated Values (.csv), 18.15 KB) MD5:df84466443f53c1733987cf4a8d9f441 Primary data file for dataset ID 918420, version 1

Supplemental Files

File
Mussel Recruit Images.zip (ZIP Archive (ZIP), 593.51 MB) MD5:9beafe83afcddbe477ca3b241230192c Supplemental file for dataset ID 918420. These images were used for the measurements of mussel length and area.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1851462

Deployments

None available yet

Instruments

Camera

Supplied Name: Leica MC170

Supplied Description:

Leica MC170: camera used to photograph recruit mussel shells.

Instrument Type

Generic Name: Camera

Acronym: camera

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/311/

Generic Description:

All types of photographic equipment including stills, video, film and digital systems.

Microscope - Optical

Supplied Name: Leica M125

Supplied Description:

Leica M125: dissecting microscope used to photograph recruit mussel shells.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

scale

Supplied Name: Mettler Toledo XP2U

Supplied Description:

Mettler Toledo XP2U: Microbalance used to measure shell dry weight.

Instrument Type

Generic Name: scale

Acronym: balance

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Mussel_Number	Mussel number.	unitless	sample
Mussel_Type	Recruit mussel shell thickness was determined for three mussel types: (1) Mytilus trossulus (from Bob Creek, Oregon), (2) M. californianus collected from Bodega Marine Reserve (BMR), and (3) M. californianus collected from Soberanes Point (SBR).	unitless	sample_descrip
Site_Latitude	Latitude of the mussel collection site.	decimal degrees	lat
Site_Longitude	Longitude of the mussel collection site (negative values = West).	decimal degrees	lon
Weight	Weight of both mussel valves.	grams (g)	weight
Magnification	Magnification setting of Leica M125 dissecting microscope.	unitless	sampling_method
Length_L	Length of left mussel valve.	millimeters (mm)	length
Length_R	Length of right mussel valve.	millimeters (mm)	length
Area_L	Projected area of left mussel valve.	square millimeters (mm^2)	area
Area_R	Projected area of right mussel valve.	square millimeters (mm^2)	area
Total_Area	Total projected area of both mussel valves.	square millimeters (mm^2)	area
Shell_Thickness	Recruit mussel shell thickness; measured as total dry weight of both valves divided by the total projected area of both valves.	grams per square millimeter (g/(mm^2))	height
Notes	Notes about mussel number taken during estimate of shell thickness.	unitless	comment