Dataset: AE1913 Clio pigments
Deployment: AE1913

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/926546

Principal Investigator:

Mak A. Saito (Woods Hole Oceanographic Institution, WHOI)

Scientist:

Natalie Cohen (Woods Hole Oceanographic Institution, WHOI)

Rodney J. Johnson (Bermuda Institute of Ocean Sciences, BIOS)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Direct Characterization of Adaptive Nutrient Stress Responses in the Sargasso Sea using Protein Biomarkers and a Biogeochemical AUV (Nutrient Stress Responses and AUV Clio)

Version:

Deployment Synonyms:

Clio-BATS-WHOI, AE1913

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Description

See the "Related Datasets" section on this page for the ELOG from cruise AE1913 and other closely related data.
* Also see all datasets related to the same cruise AE1913: https://www.bco-dmo.org/deployment/916412

Methods & Sampling

Dataset acquisition description

Oceanographic samples were collected onboard the R/V Atlantic Explorer between June 16th - 28th 2019, along a transect beginning at the Bermuda Atlantic Time-series Study (BATS) site and terminating in northeast US continental shelf waters (Woods Hole Oceanographic Institution [WHOI]). Biomass was collected using the biogeochemical AUV Clio. Clio payloads contained SUPR cartridges housing 142 mm combusted GFF filters for pigment collection. GFF filter fractions were additionally preserved for high performance liquid chromatography pigment analyses throughout the surface transect.

Pigments were collected onto 142 mm GFF filters by AUV Clio at the same depths that proteins were collected on Supor filters. GFF 25 mm punchouts were used for the pigment analysis, and the estimated volume filtered through 25 mm was used to quantify pigments per volume. Concentrations are reported as ng/kg assuming the standard density of seawater (1025 kg/m3). At stations 1 and 3, GFF fractions for pigments were not collected by Clio. Instead, pigments were analyzed from the CTD rosette at these stations (though deployments and recoveries were the same) whereby samples (4 L) were filtered directly onto 25 mm GFF glass fiber filters and immediately frozen in liquid nitrogen. Samples were analyzed on an Agilent 1100 series high performance liquid chromatography (HPLC) using the method of Bidigare et al. 2005 and instrument calibration was performed with certified pigment standards from the Danish Hydraulic Institute.

Abbreviation,Pigment Name:

ChlC3, Chlorophyll C3
ChlC2, Chlorophyll C2
Per, Peridinin
NBut, 19'But-Fucoxanthin
Fux, Fucoxanthin
NHex, 19'Hex-Fucoxanthin
Prx, Prasinoxanthin
Vix, Violoxanthin
Ddx, Diadinoxanthin
Alx, Alloxanthin
Dtx, Diatoxanthin
Lut, Lutein
Zex, Zeaxanthin
ChlB, Chlorophyll b
DvA, Divinyl Chlorophyll a
Chla, Chlorophyll a
ABCar, Alpha & Beta Carotene

Data Processing Description

Dataset Processing Description

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1658030	NSF Division of Ocean Sciences

Instruments

AUV Clio

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: AUV Clio

Generic Description:

Clio is an autonomous underwater vehicle (AUV) created to accomplish the dual goals of global ocean mapping and biochemistry sampling. The ability to sample dissolved and particulate seawater biochemistry across ocean basins while capturing fine-scale biogeochemical processes sets it apart from other AUVs. Clio is designed to efficiently and precisely move vertically through the ocean, drift laterally to observe water masses, and integrate with research vessel operations to map large horizontal scales up to a depth of 6,000 meters. More information is available at https://www2.whoi.edu/site/deepsubmergencelab/clio/

High-Performance Liquid Chromatograph

Supplied Name: Agilent 1100 series high performance liquid chromatography (HPLC)

Supplied Description:

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Niskin bottle

Supplied Name: X-Niskin bottles

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
SampleName	Sample identifier (e.g. CLIO_020_10) which includes the dive (station) and the pump filter number.	unitless	sample
Latitude	latitude. Obtained from the trace metal rosette	decimal degrees	lat
Longitude	longitude. Obtained from the trace metal rosette.	decimal degrees	lon
Depth	sample depth	meters (m)	depth
pigment_abbr	Pigment abbreviation (See "Methods & Sampling" for list of abrreviations and full pigment name).	unitless	sample_descrip
pigment_conc	Pigment concentration	nanograms per kilogram (ng/kg)	pigment_conc

Database

Contribute Data

Dataset: AE1913 Clio pigments
Deployment: AE1913

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: AE1913 Clio pigmentsDeployment: AE1913

Dataset acquisition description

Dataset Processing Description

Dataset: AE1913 Clio pigments
Deployment: AE1913