Dataset: CliOMZ Heterotrophic production rates

Name: Heterotrophic Production Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project)
Published: 2025-01-13
Keywords: oceans

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Spatial Extent: N:-90.0002 E:9.181 S:-101.4507 W:-9.9999

Eastern Tropical and Subtropical Pacific Ocean

Temporal Extent: 2023-05-04 - 2023-05-28

Project:

Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories (CliOMZ)

Principal Investigator:

Alyson E. Santoro (University of California-Santa Barbara, UCSB)

Scientist:

Barbara Bayer (University of Vienna)

Contact:

Barbara Bayer (University of Vienna)

Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2025-01-13

Restricted:

Validated:

Current State:

Preliminary and in progress

Heterotrophic Production Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project)

Abstract:

These data include heterotrophic production rates measured on R/V Atlantic (CliOMZ AT50-10 expedition) from Golfito, Costa Rica to San Diego, USA in May-June 2023. Instruments used were a CTD profiler and a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR).

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Methods & Sampling

Discrete water samples were collected using a rosette sampler equipped with 24×10 L Niskin bottles. Different depths were sampled ranging from 55 meters to 1500 meters. Water was dispensed into 2 mL microcentrifuge tubes in the laboratory. Microbial heterotrophic production was measured via the incorporation of [3H]-leucine into microbial biomass using a modified version of the microcentrifuge method (Baetge et. al., 2021). [3H]-leucine (specific activity 44.9 Ci mmol-1; Perkin Elmer) was added to 1.7 mL of sample at a final concentration of 20 nM and incubated for 2-3 h at in situ temperature. For each depth and station, triplicate live incubations and one killed control, to which 100 µL of trichloroacetic acid (TCA) was added immediately, were carried out. Incubations were terminated by addition of cold 100 µL 100% TCA and stored at 4°C until extraction following established procedures (Baetge et. al., 2021). DPM were measured on a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR) for 2 min, and [3H]-leucine incorporation rates were converted to units of carbon using a conversion factor of 1.5 kg C (mol leucine incorporated)-1 (Simon and Azam 1989).

Problems/Issues

No problems or issues reported from the dataset authors.

Data Processing Description

The mean DPM of the samples were corrected for the DPM of the blank and converted into leucine incorporation over time. Leucine incorporation rates were converted to units of carbon using a conversion factor of 1.5 kg C (mol leucine incorporated)-1 (Simon and Azam 1989).

BCO-DMO Processing

- Converted the primary data file from a variable width plain text file to a CSV file.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1924512

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AT50-10	CliOMZ	2023-05-02	R/V Atlantis	Mak A. Saito (Chief Scientist) Alyson E. Santoro (Chief Scientist)	data info

Instruments

Liquid Scintillation Counter

Supplied Name: Perkin-Elmer Tri-Carb 2910 TR

Supplied Description:

Disintegrations per minute (DPM) were counted in a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR).

Instrument Type

Generic Name: Liquid Scintillation Counter

Acronym: LSC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Generic Description:

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample.

Niskin bottle

Supplied Name: 24x10 L Niskin Bottles

Supplied Description:

Discrete water samples were collected using a rosette sampler equipped with 24×10 L Niskin bottles.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

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Dataset: CliOMZ Heterotrophic production rates