Dataset: Niskin bottle samples
Deployment: KM1910

Niskin bottle samples

Principal Investigator:

Matthew J. Church (University of Montana)

Co-Principal Investigator:

Sara Ferrón (University of Hawaiʻi at Mānoa, SOEST)

Erica Goetze (University of Hawaiʻi at Mānoa, SOEST)

Angelicque E. White (University of Hawai'i, UH)

Contact:

Ashley Maloney (Princeton University)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

EAGER Collaborative Research: Early career chief scientist training for biological and chemical oceanographers (Chief Sci KM1910)

Deployment Synonyms:

EAGER, Chief Sci cruise, 2019 Chief Scientist Training Cruise

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/852183/data

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Description

Particulate phosphate, particulate carbon, particulate nitrogen, particulate bulk isotope composition (C and N), and dissolved oxygen, collected with Niskin bottles during CTD deployments in June 2019 at station ALOHA during cruise KM1910.

Methods & Sampling

Dataset acquisition description

CTD and Niskin sampling follow HOT procedures summarized in detail: https://hahana.soest.hawaii.edu/hot/protocols/protocols.html#

Dissolved Oxygen sampling and titrations from Niskin bottles follow HOT procedures. Briefly, oxygen samples were the first samples drawn from the Niskin bottles as soon as the rosette arrived on deck, free of bubbles, and fixed. Samples were analyzed with a computer controlled potentiometric titration. At 2 depths on each cast, 3 titrations were performed and had an average standard deviation of 0.4 micromol/L.

Particulate Carbon and Nitrogen field and laboratory protocols follow HOT procedures (except sample analysis took place at Princeton University using a Vario ISOTOPE select EA-IRMS). Briefly, 4 to 20 L of water was collected from Niskin bottles, pre-filtered with an inline 202um mesh screen, and collected on combusted 25mm GF/F (Whatman, nominal pore size 0.7um), folded, and wrapped in clean (combusted) foil for storage at -20 degrees C until laboratory analysis. Samples were oven dried at 55-60 degrees celsius (samples were not acidified so C represents total C), pelletized in 9x11 tins, and analyzed via EA-IRMS for particulate carbon, delta-13C particulate carbon, particulate nitrogen, delta-15N particulate nitrogen. Six blank (dry) filters were analyzed for background C and N and subtracted from measured PC and PN sample values.

Particulate Phosphate field and laboratory protocols follow HOT procedures. Briefly, 4 to 10 L of water was collected from Niskin bottles, pre-filtered with an inline 202um mesh screen, and collected on combusted 25mm GF/F (Whatman, nominal pore size 0.7um), folded, and placed in clean glass tube, storage at -20 degrees C until laboratory analysis. One sample from each depth was combusted in 16 x 100 mm clean glass test tubes at 450°C for 4.5 hours in a muffle furnace, allowed to cool and immersed in 10ml 0.15N HCL/vortex/ 1 hour leach/vortex/30 Minute Spin/ 1 hour color development) (5ml sample : 500ul Mixed Reagent). The liberated orthophosphate is reacted with a mixed reagent of molybdic acid, ascorbic acid and trivalent antimony to form phosphomolybdic acid. This heteropoly acid is then reduced to the colored molybdenum blue complex by ascorbic acid and the solution is measured spectrophotometrically. This procedure measures all forms of phosphorus which can be released by combustion and acid hydrolysis. Three blank (dry) filters were analyzed for background P and subtracted from measured PP sample values.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:
- converted date_HST to YYYY-MM-DD format;
- created UTC date/time field in ISO8601 format.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1911831	NSF Division of Ocean Sciences
OCE-1911990	NSF Division of Ocean Sciences

Instruments

Automatic titrator

Supplied Name: Dosimat titrator (Brinkmann)

Supplied Description:

5 ml Dosimat titrator (Brinkmann)

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

CTD Sea-Bird 911

Supplied Name: Sea-Bird SBE 911

Supplied Description:

The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.)

Instrument Type

Generic Name: CTD Sea-Bird 911

Acronym: CTD SBE 911

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/

Generic Description:

Isotope-ratio Mass Spectrometer

Supplied Name: Vario ISOTOPE select (Elementar Isoprime)

Supplied Description:

A Vario ISOTOPE select (Elementar Isoprime) was used to quantify total C, N, and delta-13C and delta-15N. Samples are combusted/reduced at high temperature to create gases (CO2 and N2); column chromatography separates the gases; a thermal conductivity detector determines the carbon and nitrogen concentrations, followed by an isotope ratio mass spectrometer for bulk isotope composition. Measurements were calibrated with an in-house aminocaproic acid standard (ACROS) and four USGS standards (#41, #41a, #65, #25). [C] and [N] (umol/L) were corrected for the average value measured on blank filters (n=6). Analytical uncertainty (determined from standards with similar isotope values) was 0.2‰ for delta-15N and 0.1‰ for delta-13C.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

muffle furnace

Supplied Description:

One sample from each depth was combusted in 16 x 100 mm clean glass test tubes at 450°C for 4.5 hours in a muffle furnace, allowed to cool and immersed in 10ml 0.15N HCL/vortex/ 1 hour leach/vortex/30 Minute Spin/ 1 hour color development) (5ml sample : 500ul Mixed Reagent).

Instrument Type

Generic Name: muffle furnace

Generic Description:

A muffle furnace or muffle oven (sometimes retort furnace in historical usage) is a furnace in which the subject material is isolated from the fuel and all of the products of combustion, including gases and flying ash. A type of jacketed enclosure that is used to heat a material to significantly high temperatures while keeping it contained and fully isolated from external contaminants, chemicals or substances. Muffle furnaces are usually lined with stainless steel, making them largely corrosion-resistant.

Niskin bottle

Supplied Description:

Briefly, a Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles were deployed in a 24 bottle rosette system mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	Cruise identifier	unitless	cruise_id
sta	Station number	unitless	station
cast	CTD cast identifier	unitless	cast
lon	Longitude (degrees E)	degrees East	lon
lat	Latitude (degrees N)	degrees North	lat
date_HST	Local date (HST) of deployment; format: YYYY-MM-DD	unitless	date_local
time_HST	Local time (HST) of CTD recovery (24 hour time); format: hhmm	unitless	time_local
ISO_DateTime_UTC	Date and time (UTC) of CTD recovery in ISO8601 format: YYYY-MM-DDThh:mmZ	unitless	ISO_DateTime_UTC
targetdepth_m	Target depth of sample in meters (m)	meters (m)	depth
pressure_mrk	Pressure at collection depth in decibar from CTD .mrk file	decibars	press
sal_mrk	Salinity at collection depth from CTD .mrk file	PSU	sal
bottle	Niskin bottle number(s) (position(s) on rosette)	unitless	bottle
oxygen	Dissolved oxygen (umol/L), average uncertainty = 0.4 umol/L determined from triplicate titrations at 2 depths from each cast	micromoles per liter (umol/L)	O2
PP	Particulate Phosphate (PP) [umol/L] (4 to 10 L sample prefiltered with 202um mesh screen onto combusted 25mm GF/F; measurements corrected for 3 blank (dry) filters)	micromoles per liter (umol/L)	PO43-
samplesizePCPN_L	Liters of water taken from bottle(s) (deepest depths combined 2 bottles)	liters (L)	sample_volume
PC	Total Particulate Carbon (PC) [umol/L] (prefiltered with 202um mesh screen onto combusted 25mm GF/F; filters not acidified before analysis; measurements corrected for 6 blank (dry) filters)	micromoles per liter (umol/L)	TPC
P13C	Delta-13C of total PC (permil vs. VPDB) analytical uncertainty 0.1 permil (prefiltered with 202um mesh screen onto combusted 25mm GF/F; filters not acidified before analysis)	permil vs VPDB	d13C
PN	Particulate Nitrogen (PN) [umol/L] (prefiltered with 202um mesh screen onto combusted 25mm GF/F; filters not acidified before analysis; measurements corrected for 6 blank (dry) filters). Detection limit ~0.75 micromol N.	micromoles per liter (umol/L)	N
P15N	Delta-15N of PN (permil vs. air-N2) analytical uncertainty 0.2 permil (prefiltered with 202um mesh screen onto combusted 25mm GF/F; filters not acidified before analysis)	permil vs air-N2	d15N

Database

Contribute Data

Dataset: Niskin bottle samples
Deployment: KM1910

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Niskin bottle samplesDeployment: KM1910

Dataset acquisition description

Dataset Processing Description

Dataset: Niskin bottle samples
Deployment: KM1910