Dataset: Ultrahigh Resolution Mass Spectrometry of a Depth Profile
Deployment: KM2110

Ultrahigh Resolution Mass Spectrometry of a Depth Profile

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/905077

Principal Investigator:

Michael Gonsior (University of Maryland Center for Environmental Science, UMCES/CBL)

Co-Principal Investigator:

Feng Chen (University of Maryland Center for Environmental Science, UMCES/IMET)

Contact:

Michael Gonsior (University of Maryland Center for Environmental Science, UMCES/CBL)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

The fate of lysis products of picocyanobacteria contributes to marine humic-like chromophoric dissolved organic matter (Picocyanobacteria CDOM)

Version:

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Description

Methods & Sampling

Dataset acquisition description

Water samples were collected using the ship's CTD profiler and 12 L Nisking bottles. 10 L water samples from each depth were then transferred into 5 gallon polycarbonate water bottles and extractrated. 10 L seawater samples were collected at 200 m depth intervals and then solid-phase extracted suing Agilent Bond Elut PPL cartridges (1 g, 6 ml). The organic matter was then eluted with 10 mL methanol. The methanolic extract was diluted 1:20 and directly infused into a 12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer. 500 scans were averaged and mass lists were calibrated at a mass accuracy of greater than 0.2 ppm.

Data Processing Description

Dataset Processing Description

Data were calibrated using known DOM signatures and a matrix was generated aligning all spectra. See Timko et al., 2015 Reference for the calibration of FT-ICR MS data.

BATS depth 120m = deep chlorophyll maximum (DCM)
BATS depth 820m = oxygen minimum zone (OMZ)

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1829888	NSF Division of Ocean Sciences

Instruments

Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Supplied Name: 12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer

Supplied Description:

Instrument Type

Generic Name: Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Acronym: FTICR MS

Generic Description:

In Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, the mass-to-charge ratio (m/z) of an ion is experimentally determined by measuring the frequency at which the ion processes in a magnetic field. These frequencies, which are typically in the 100 KHz to MHz regime, can be measured with modern electronics making it possible to determine the mass of an ion to within +/- 0.000005 amu or 5 ppm.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Station_Location	Sampling station (HOT or BATS)	unitless	station
Latitude	Latitude sampling location, south is negative	decimal degrees	lat
Longitude	Longitude sampling location, west is negative	decimal degrees	lon
Date	Sampling date	unitless	date
Replicate	Replicate number	unitless	replicate
Depth	Sampling Depth	meters (m)	depth
Mass	Mass to charge ratio (m/z)	unitless	mass_to_charge
Intensity	Ion intensity (arbitrary unit)	unitless	intensity

Database

Contribute Data

Dataset: Ultrahigh Resolution Mass Spectrometry of a Depth Profile
Deployment: KM2110

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Ultrahigh Resolution Mass Spectrometry of a Depth ProfileDeployment: KM2110

Dataset acquisition description

Dataset Processing Description

Dataset: Ultrahigh Resolution Mass Spectrometry of a Depth Profile
Deployment: KM2110