Dataset: DMS_DMSP_Ant1
Deployment: NBP0601

Dimethylsulfide (DMS) and Dymethylsulfonoiproprionate (DMSP) from temp and iron experiments on Antarctic phyto and microzooplankton

Principal Investigator:

Dr Julie Rose (National Oceanic and Atmospheric Administration , NOAA-Milford)

Co-Principal Investigator:

David A. Hutchins (University of Southern California, USC)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Controls of Ross Sea Algal Community Structure (CORSACS)

Rising climatic temperatures impact on antarctic microzooplankton growth and grazing (Antarctic microzooplankton )

Deployment Synonyms:

CORSACS I

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/505528/data

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Description

Experimental Design

Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in January 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0601). Water was collected at 75.00S, 177.36E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Water was prescreened through acid-washed 200μm Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 12 4.5-L and 12 2.7-L acid washed trace metal clean clear polycarbonate bottles for incubation. Half of the bottles were spiked with 1.0nM FeCl3 (final concentration) at the beginning of the experiment. Bottles were incubated in two temperature controlled deck-board incubators (Feng et al., 2009; Hare et al., 2007). Incubators were screened to 18% of Io using two layers of neutral density filter. One incubator was kept at ambient temperature (0 deg C), while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for seven days. The 4.5-L bottles were sampled daily and the 2.7-L bottles were only sampled on the final day of the experiment. All sampling occurred under a laminar flow hood using trace metal clean techniques.

References

Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.

Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.

Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.

Methods & Sampling

Dataset acquisition description

DMS/DMSP

Samples for dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) were collected using the methodology of Kiene and Slezak (2006). A small volume of each sample (≤15 mL) was gravity filtered through a 25mm Whatman GF/F filter and the filtrate collected for the immediate determination of DMS. A second gravity-filtered sample (<eq;20 mL) was collected and the filtrate preserved with 50% sulfuric acid for the determination of dissolved DMSP (DMSPd). Aliquots of the unfiltered samples (≤20 mL) were preserved with 50% sulfuric acid (100uL per 10mL of sample) for the determination of total DMSP (DMSPt). All DMSP samples were stored at 0oC until they could be analyzed, which was within three days of their collection. Upon analysis, the DMSP samples were base-hydrolyzed (2 mol L−1 sodium hydroxide) and measured as DMS using a cryogenic purge and trap systems coupled to either a Hewlett-Packard 5890 Series II gas chromatograph and an Agilent 6890 gas chromatograph that were fitted with flame photometric detectors (DiTullio and Smith, 1995). Both systems were calibrated using constant-temperature DMS permeation devices (Vici Metronics) and DMSP standards (Research Plus Inc). Particulate DMSP (DMSPp) was calculated as the difference between DMSPt and DMSPd.

References

DiTullio, G.R., and W.O. Smith, Jr. 1995. Relationship between dimethylsulfide and phytoplankton pigment concentrations in the Ross Sea, Antarctica. Deep-Sea Research I 42: 873-892.

Kiene, R.P., and D. Slezak. 2006. Low dissolved DMSP concentrations in seawater revealed by small-volume gravity filtration and dialysis sampling. Limnology and Oceanography-Methods 4: 80-95.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing Notes:

- File was sorted by treatment
- Added lat,lon values of original water sampling location to file
- Added BCO-DMO header lines
- Parameter names were edited to conform with BCO-DMO convention

More information about this dataset deployment

Funding

Award Number	Funding Source
PLR-0528715	NSF Antarctic Sciences

Instruments

Gas Chromatograph

Supplied Description:

A cryogenic purge and trap systems coupled to either a Hewlett-Packard 5890 Series II gas chromatograph and an Agilent 6890 gas chromatograph that were fitted with flame photometric detectors (DiTullio and Smith, 1995) were used to determine DMS and DMSP.

Instrument Type

Generic Name: Gas Chromatograph

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/

Generic Description:

Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
lat	Latitude component of geographic position where water was sampled.	decimal degrees	lat
lon	Longitude component of geographic position where water was sampled.	decimal degrees	lon
treatment	Experimental conditions varied during the experiment. Four treatments were used: High temperature, high iron (HTHF); High temperature, low iron (HTLF); Low temperature, high iron (LTHF); Low temperature, low iron (LTLF)	dimensionless	treatment
day	Sampling day during experiment. The experiment was conducted during January, 2006.	dimensionless	no_bcodmo_term
bottle	Experimental bottle number.	dimensionless	no_bcodmo_term
DMS	Dimethyl sulphide.	nanomoles per Liter	DMS
DMSP_disslv	Dissolved dimethylsulphoniopropionate.	nanomoles per Liter	DMSP
DMSP_total	Total dimethylsulphoniopropionate.	nanomoles per Liter	DMSP
DMSP_partic	Particulate dimethylsulphoniopropionate.	nanomoles per Liter	DMSP

Database

Contribute Data

Dataset: DMS_DMSP_Ant1
Deployment: NBP0601

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: DMS_DMSP_Ant1Deployment: NBP0601

Dataset acquisition description

Dataset Processing Description

Dataset: DMS_DMSP_Ant1
Deployment: NBP0601