Dataset: Auto-induction in Marine Diatoms
Deployment: Lab_UCSD_Vernet

Results from laboratory experiments testing autoinduction in marine diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum

Principal Investigator:

Dr Maria Vernet (University of California-San Diego Scripps, UCSD-SIO)

Data Manager:

Ms Dicky Allison (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

BCO-DMO Data Manager:

Ms Dicky Allison (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Cell to cell communication in marine phytoplankton: population density control of cellular processes (PhytoplanktonQS)

Current State:

Final no updates expected

Version:

data collection complete

Version Date:

2014-05-14

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/514891/data

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Description

Data from laboratory experiments testing the effect of auto-induction on the marine diatorms Thalassiosira pseudonana and Phaeodactylum tricornutum.

Methods & Sampling

Dataset acquisition description

Laboratory experiments were conducted to test auto-induction in marine diatoms. Experiments were conducted including initial tests to establish a reliable and repeatable protocol at each step of the experimental design. All findings are based on triplicate experiments, i.e. each treatment in triplicate.

For further details of Methods and Analytical Methodology, please contact the PI.

Data Processing Description

Dataset Processing Description

Data were plotted; points above or below one standard deviation were considered outliers. Averages and Standard Deviations were calculated from Excel.

Quality control: Several experimental designs failed and are not included. Only data of publishable quality are included in the data file.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1140042	NSF Division of Ocean Sciences

Instruments

In-situ incubator

Supplied Name: Percival models LT-36VL and LT-41VLX

Supplied Description:

Instrument Type

Generic Name: In-situ incubator

Acronym: in-situ incubator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/82/

Generic Description:

A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.

pH Sensor

Supplied Name: ThermoScientific Orion Star LogR

Supplied Description:

Accuracy to 2 decimal places.

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Spectrophotometer

Supplied Name: Cary-100 UV-Visible spectrophotometer

Supplied Description:

Accurate to 4 decimal places

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
species	specific taxonomic classification	none	species
expt_num	Sequential numbering system for each part of the experiment.	none	exp_id
date_local	day/month/year of the experiment	dd/mm/yy	date_local
samp_name	FM = Fresh Medium; CM = Conditioned Medium; Because the display does not support '(prime) and "(double prime), those annotations were changed to 'prm' and 'dblprm'.	none	sample
cell_conc	concentration of cells in the sample at the beginning of the experiment.	cells per milliliter	cell_concentration
OD_blank	Optical Density of the blank at 750 nm. This dataset has three replicate measurements of Optical density blanks: OD_blank1, OD_blank2 and OD_blank3. They correspond to three measurements of the Optical density of the diatom concentration and set the location of the instrument zero.	optical density units	instrument_blank
OD_blank_avg	Average of three blanks measured by the spectrophotometer.	optical density units	instrument_blank
OD	Optical Density (OD) of the sample at 750 nm. OD1, OD2 and OD3 are replicate measurements.	optical density units	optical_density
OD_avg_corr	Corrected OD average of the sample: OD sample minus OD blank	optical density units	optical_density
avg_OD_triple	Average of the three replicate sample measurements: first, prime and doubleprime.	optical density units	optical_density
OD_std_dev	Standard deviation of the triples measurements.	optical density units	optical_density
pH	pH of the sample	pH units	pH

Database

Contribute Data

Dataset: Auto-induction in Marine Diatoms
Deployment: Lab_UCSD_Vernet

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Auto-induction in Marine DiatomsDeployment: Lab_UCSD_Vernet

Dataset acquisition description

Dataset Processing Description

Dataset: Auto-induction in Marine Diatoms
Deployment: Lab_UCSD_Vernet