Dataset: Radioisotopic rate measurements from the Cariaco Basin
Deployment: CAR212_2

Radioisotope data from the CAR212 and CAR216 cruises.

Principal Investigator:

Virginia P. Edgcomb (Woods Hole Oceanographic Institution, WHOI)

Gordon T. Taylor (Stony Brook University - SoMAS, SUNY-SB SoMAS)

Co-Principal Investigator:

Dr Craig Taylor (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Genetic and Metabolic Signatures of Marine Microorganisms in Oxygen Depleted and Varying Geochemical Seascapes (CariacoMetaOmics)

Current State:

Final with updates expected

Version:

final

Version Date:

2016-07-22

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/652505/data

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Description

Dark DIC assimilation and bacterial net productivity data measured from incubation experiments on 1 leg of each of the CAR212 and CAR216 cruises.

Methods & Sampling

Dataset acquisition description

All samples were collected via Niskin bottles.

Dark DIC assimilation, a proxy for chemoautotrophy, and bacterial net productivity, BNP, were determined by incorporation of 14C- bicarbonate and 3H-leucine into microbial biomass, respectively, according to Taylor et al. (2001) and Kirchman (1993). Incubations were done at in situ temperatures and oxygen concentrations. After incubations were terminated, the microbial biomass was collected onto filters, stored in scintillation cocktail (Hionic-Fluor, Perkin Elmer) and activity was measured on a Tri-Carb 2810TR (Perkin Elmer) scintillation counter.

Data Processing Description

Dataset Processing Description

All data were normalized to volume of seawater.

DMO Notes:

-transferred radioisotope data section into separate a data object.
-reformatted the date to comply with BCO-DMO standards.
-reformatted some column names to comply with BCO-DMO standards.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1335436	NSF Division of Ocean Sciences
OCE-1336082	NSF Division of Ocean Sciences

Instruments

Liquid Scintillation Counter

Supplied Name: Tri-Carb 2810TR (Perkin Elmer) scintillation counter

Supplied Description:

Activity was measured on this scintillation counter

Instrument Type

Generic Name: Liquid Scintillation Counter

Acronym: LSC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Generic Description:

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Niskin bottle

Supplied Description:

All samples were collected via Niskin bottles.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	cariaco cruise number and leg number	unitless	cruise_id
date_start	start date; mm/dd/yyyy	unitless	date_start
depth	depth	meters	depth
dark_DIC_assim	dark DIC assimilation	micrograms of C per liter per day	biomass_C
dark_DIC_assim_sd	standard deviation of the dark DIC assimilation	micrograms of C per liter per day	std_dev
bac_net_productivity	bacterial net productivity by leucine incorporation	micrograms of C per liter per day	biomass_C
bac_net_productivity_sd	standard deviation of the bacterial net productivity by leucine incorporation	micrograms of C per liter per day	std_dev

Database

Contribute Data

Dataset: Radioisotopic rate measurements from the Cariaco Basin
Deployment: CAR212_2

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Radioisotopic rate measurements from the Cariaco BasinDeployment: CAR212_2

Dataset acquisition description

Dataset Processing Description

Dataset: Radioisotopic rate measurements from the Cariaco Basin
Deployment: CAR212_2