Dataset: Radioisotopic rate measurements from the Cariaco Basin

Name: Radioisotope data from B/O Hermano Gines cruises in the CARIACO Basin Time Series Station from May to November 2014 (CariacoMetaOmics project)
Published: 2016-07-22
Keywords: oceans

Cite This Dataset

Project:

Genetic and Metabolic Signatures of Marine Microorganisms in Oxygen Depleted and Varying Geochemical Seascapes (CariacoMetaOmics)

Principal Investigator:

Virginia P. Edgcomb (Woods Hole Oceanographic Institution, WHOI)

Gordon T. Taylor (Stony Brook University - SoMAS, SUNY-SB SoMAS)

Co-Principal Investigator:

Dr Craig Taylor (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

final

Version Date:

2016-07-22

Restricted:

Validated:

Yes

Current State:

Final with updates expected

Radioisotope data from B/O Hermano Gines cruises in the CARIACO Basin Time Series Station from May to November 2014 (CariacoMetaOmics project)

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Description

Dark DIC assimilation and bacterial net productivity data measured from incubation experiments on 1 leg of each of the CAR212 and CAR216 cruises.

Methods & Sampling

All samples were collected via Niskin bottles.

Dark DIC assimilation, a proxy for chemoautotrophy, and bacterial net productivity, BNP, were determined by incorporation of 14C- bicarbonate and 3H-leucine into microbial biomass, respectively, according to Taylor et al. (2001) and Kirchman (1993). Incubations were done at in situ temperatures and oxygen concentrations. After incubations were terminated, the microbial biomass was collected onto filters, stored in scintillation cocktail (Hionic-Fluor, Perkin Elmer) and activity was measured on a Tri-Carb 2810TR (Perkin Elmer) scintillation counter.

Data Processing Description

All data were normalized to volume of seawater.

DMO Notes:

-transferred radioisotope data section into separate a data object.
-reformatted the date to comply with BCO-DMO standards.
-reformatted some column names to comply with BCO-DMO standards.

Data Files

File
Radioisotope_rate.csv (Comma Separated Values (.csv), 1.61 KB) MD5:c051dfb75b0fe2966eee34984c4e4aff Primary data file for dataset ID 652461

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1335436
NSF Division of Ocean Sciences	OCE-1336082

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
CAR212_2		2014-05-07	B/O Hermano Gines		data info
CAR216_2		2014-11-05	B/O Hermano Gines		data info

Instruments

Liquid Scintillation Counter

Supplied Name: Tri-Carb 2810TR (Perkin Elmer) scintillation counter

Supplied Description:

Activity was measured on this scintillation counter

Instrument Type

Generic Name: Liquid Scintillation Counter

Acronym: LSC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Generic Description:

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample.

Niskin bottle

Supplied Description:

All samples were collected via Niskin bottles.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	cariaco cruise number and leg number	unitless	cruise_id
date_start	start date; mm/dd/yyyy	unitless	date_start
depth	depth	meters	depth
dark_DIC_assim	dark DIC assimilation	micrograms of C per liter per day	biomass_C
dark_DIC_assim_sd	standard deviation of the dark DIC assimilation	micrograms of C per liter per day	std_dev
bac_net_productivity	bacterial net productivity by leucine incorporation	micrograms of C per liter per day	biomass_C
bac_net_productivity_sd	standard deviation of the bacterial net productivity by leucine incorporation	micrograms of C per liter per day	std_dev

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Database

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Dataset: Radioisotopic rate measurements from the Cariaco Basin