Dataset: Grazing experiment 2 and 3: pCO2
Deployment: Lab_Olson_B

Carbonate chemistry data for low-high pCO2 acclimated E. huxleyi cultures

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kasey Kendall (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/661395/data

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Description

Related Reference:
Kendall, K., Marine Microzooplankton are Indirectly Affected by Ocean Acidification Through Direct Effects on Their Phytoplankton Prey. (Masters Thesis) Western Washington University.
http://cedar.wwu.edu/wwuet/448/

Methods & Sampling

Dataset acquisition description

The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for pH were filtered through a GFF to remove cells and loose coccoliths both of which increase optical scatter. Samples were warmed to 25°C in a water bath and run within three hours of sampling. pH was analyzed spectrophotometrically with m-cresol dye on an Agilent 8453A UV-VIS Diode Array Spectrophotometer. Samples for total Alkalinity were taken on growth days 5, 7 and 10. Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel (+/1 5 ueq/kg). Other parameters were calculated with CO2sys

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- column names reformatted to comply with BCO-DMO standards
- nd (no data) was entered into all blank cells

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961229	NSF Division of Ocean Sciences

Instruments

Automatic titrator

Supplied Name:

Supplied Description:

Titrando 888

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Spectrophotometer

Supplied Name:

Supplied Description:

Agilent 8453A UV-VIS Diode Array Spectrophotometer

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
treatment_rep_culture_day	pCO2 level (ambient; moderate; high) replicate (A;B;C) and day of semi-continuous culture	unitless	treatment
pH	pH of the culture material removed	pH scale	pH
pCO2	Partial pressure of carbon dioxide (pCO2) of the culture material removed; computation from pH and alkalinity	parts per million by volume (ppmv)	pCO2
total_alkalinity	total alkalinity of the culture material removed	micromoles per kilogram (umol/kg)	TALK

Database

Contribute Data

Dataset: Grazing experiment 2 and 3: pCO2
Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Grazing experiment 2 and 3: pCO2Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Dataset: Grazing experiment 2 and 3: pCO2
Deployment: Lab_Olson_B