Dataset: Particulate vitamin B12 profiles
Deployment: KM1314

Particulate vitamin B12 profiles from cruise KM1314, Aug. 8 - Sept. 4, 2013 in the northern Pacific

Principal Investigator:

Dr David A. Stahl (University of Washington, UW)

Co-Principal Investigator:

E. Virginia Armbrust (University of Washington, UW)

Allan Devol (University of Washington, UW)

Anitra E. Ingalls (University of Washington, UW)

James W. Moffett (University of Southern California, USC)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Significance of nitrification in shaping planktonic biodiversity in the ocean (Nitrification and Marine Planktonic Biodiversity)

Version Date:

2017-01-04

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/672372/data

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Description

Particulate vitamin B12 data from R/V Kilo Moana KM1314 in the North Pacific Ocean. These data are published in Heal et al (2016), Supplemental Table 2.

Related Reference:
Katherine R. Heal, Wei Qin, Francois Ribalet, Anthony D. Bertagnolli, Willow Coyote-Maestas, Laura R. Hmelo, James W. Moffett Allan H. Devol, E. Virginia Armbrust, David A. Stahl, and Anitra E. Ingalls. Two distinct pools of B12 analogs reveal community interdependencies in the ocean. (2016) PNAS. www.pnas.org/cgi/doi/10.1073/pnas.1608462114
Supplemental Information

Methods & Sampling

Dataset acquisition description

Environmental samples (particulate material > 0.2 µm) were analyzed for cobalamins and pseudocobalamins using an organic solvent extraction paired with LC-MS as described in Heal et al. 2014.

Reference:
Heal, K.R., Carlson, L.T., Devol, A.H., Armbrust, E.V., Moffett, J.W., Stahl, D.A., et al., 2014. Determination of four forms of vitamin B12 and other B vitamins in seawater by liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 28, 2398–2404. DOI: 10.1002/rcm.7040

Data Processing Description

Dataset Processing Description

BCO-DMO Data Processing Notes:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standards

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1046017	NSF Division of Ocean Sciences

Instruments

High-Performance Liquid Chromatograph

Supplied Name: HPLC (Agilent 1100) and UPLC Waters Acquity

Supplied Description:

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Mass Spectrometer

Supplied Name: a quadrupole time-of-flight mass spectrometer (Waters Xevo G2-S QTOF)

Supplied Description:

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	cruise identifier	unitless	cruise_id
sta	station number	unitless	sta
depth	sampling depth	meters	depth
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
OH_Pseudo	Hydroxopseudocobalamin concentration; dl=below detection limit	picomole (pM)	no_bcodmo_term
Me_Pseudo	Methylpseudocobalamin concentration; dl=below detection limit	picomole (pM)	no_bcodmo_term
OH_Cobalamin	Hydroxocobalamin concentration; dl=below detection limit	picomole (pM)	no_bcodmo_term
Me_Cobalamin	Methylcobalamin concentration; dl=below detection limit	picomole (pM)	no_bcodmo_term
Ado_Cobalamin	Adenosylcobalamin concentration; dl=below detection limit	picomole (pM)	no_bcodmo_term

Database

Contribute Data

Dataset: Particulate vitamin B12 profiles
Deployment: KM1314

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Particulate vitamin B12 profilesDeployment: KM1314

Dataset acquisition description

Dataset Processing Description

Dataset: Particulate vitamin B12 profiles
Deployment: KM1314