Dataset: dataset4_Krug_et_al_2012: Philine sequences
Deployment: Krug_lab

Accessions for 16S for Philine species

Principal Investigator:

Patrick Krug (California State University Los Angeles, Cal State LA)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Quantifying larval behavior to reconcile genetic connectivity with biophysical model predictions (PLDvFST)

Data URL:

https://osprey.bco-dmo.org/dataset-deployment/682253/data

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Description

Sequences were generated for 10 Philine species sampled globally. Collection information, accessions and links to the GenBank pages are provided.

Detailed collection information and archival data for specimens of Philine spp. ID codes are in-house labels. National Center for Bioinformatics (NCBI) accession numbers for unique 16S haplotypes are listed with a given haplotype. Museum voucher numbers are for the California Academy of Sciences (CASIZ), British Museum of Natural History (BMNH), or Auckland War Memorial Museum (AK). CA = California; OR = Oregon, U.S.A. Samples in parentheses were positively identified in the present study. Samples are from invaded sites are shown under 'invasion' column. Dashes indicate specimens that were not available for accessioning in a museum collection. 'Additional specimens' denotes vouchered material that was not sequenced in the present study.

Related Reference: Krug, P.J., Asif, J., Baeza, I., Morley, M., Blom, W., and T. Gosliner. 2012. Molecular identification of two species of the carnivorous sea slug Philine, invaders of the U.S. west coast. Biological Invasions, 14: 2447-2459. doi: 10.1007/s10530-012-0242-9.

Methods & Sampling

Dataset acquisition description

Purified PCR products were directly cycle-sequenced in both directions using PCR primers and Big Dye Terminator 3.1 Cycle Sequencing chemistry at the High-Throughput Genomics Unit, University of Washington or on an ABI PrismTM 377 DNA Sequencer (Applied Biosystems). Chromatograms were edited and primer sequences removed in GeneiousPro 4.8 software.

For full details on sampling and analytical methodology, see Krug et al (2012).

Data Processing Description

Dataset Processing Description

Sequences were edited to remove primer sequences.

BCO-DMO Processing notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- added links to NCBI accessions
- spelled out genus names
- replaced hyphens with 'did_not_amplify'

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1130072	NSF Division of Ocean Sciences

Instruments

Automated DNA Sequencer

Supplied Name:

Supplied Description:

Big Dye Terminator 3.1 Cycle Sequencing chemistry at the High-Throughput Genomics Unit, University of Washington or on an ABI PrismTM 377 DNA Sequencer (Applied Biosystems).

Instrument Type

Generic Name: Automated DNA Sequencer

Acronym: Automated Sequencer

Generic Description:

General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.

Thermal Cycler

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Thermal Cycler

Generic Description:

A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.

(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
specimen_id	in-house specimen identifier	unitless	sample
species	specimen identifier code	unitless	species
invasion	whether the specimens were from an invaded site or not	unitless	species
haplotype_16S	haplotype 16S identifier	unitless	no_bcodmo_term
accession_16S	16S gene GenBank accession number	unitless	accession_number
accession_16S_link	link to 16S gene GenBank accession page	unitless	external_link
voucher	museum voucher number: CASIZ = California Academy of Sciences; BMNH = British Museum of Natural History; AK = Auckland War Memorial Museum	unitless	sample
collection_locality	place of collection	unitless	site
date_collected	date collected	unitless	date
collector	collector's name	unitless	person

Database

Contribute Data

Dataset: dataset4_Krug_et_al_2012: Philine sequences
Deployment: Krug_lab

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: dataset4_Krug_et_al_2012: Philine sequencesDeployment: Krug_lab

Dataset acquisition description

Dataset Processing Description

Dataset: dataset4_Krug_et_al_2012: Philine sequences
Deployment: Krug_lab