Dataset: Phillips_2011 - Experiment 2 Larval Mortality
Deployment: Osenberg_et_al_Moorea

In this experiment, larval growth and metamorposis was tested using different food levels.

Larvae hatched on October 10, 2009 and ~50 were distributed into each tubs on 500mL filtered sea water (FSW). Because the greatest metamorphic success was in the Isochrysis galbana treatment during experiment 1, only that species was used in experiment 2.

Three food densities were created high food (4 x 104 cells mL-1 ), low food (4 x 103 cells mL-1 ) plus an Unfed treatment in which larvae were raised in FSW. Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. Each of the three treatments had three replicate containers; 50 larvae were placed into each container initially, exept two containers that had 1 extra. Every 3 days for 9 days starting on October 4, 2009, larvae were counted in each container to quantify survival. Water and food were also replenished. On days 6 and 8, fifteen larvae were transferred for the settlement challenge experiment.