Dataset: Phillips_2011 - Experiment 2 Larval Mortality

Name: Lab experiment to determine survival and mortality of Ceraesignum (formerly Dendropoma) maximum after 3, 6, and 9, days depending on amount fed in Moorea, French Polynesia from October 2009 (Vermetids_Corals project)
Published: 2017-10-05
Keywords: oceans

Cite This Dataset

Spatial Extent: N:-17.47279 E:-149.78277 S:-17.48365 W:-149.84698

Temporal Extent: 2009-10-04 - 2009-10-13

Project:

Spatial patterns of coral-vermetid interactions: short-term effects and long-term consequences (Vermetids_Corals)

Principal Investigator:

Nicole Phillips (Victoria University of Wellington)

Contact:

Nicole Phillips (Victoria University of Wellington)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2017-10-05

Version Date:

2017-10-05

Restricted:

Current State:

Preliminary and in progress

Lab experiment to determine survival and mortality of Ceraesignum (formerly Dendropoma) maximum after 3, 6, and 9, days depending on amount fed in Moorea, French Polynesia from October 2009 (Vermetids_Corals project)

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Description

These data are from an experiment that test the nutritional strategies of Ceraesignum (Dendropoma) maximum larvae. For additional datasets see related files.

Related Datasets:

Phillips_2011 - Experiment 1 Larval Mortality: https://www.bco-dmo.org/dataset/725276
Phillips_2011 - Experiment 1 Larval Size: https://www.bco-dmo.org/dataset/725317
Phillips_2011 - Experiment 1 Settlement Challenge 10: https://www.bco-dmo.org/dataset/725335
Phillips_2011 - Experiment 1 SettlementChallenge18: https://www.bco-dmo.org/dataset/725392
Phillips_2011 - Experiment 2 Larval Mortality: https://www.bco-dmo.org/dataset/725880 (Current page)
Phillips_2011 - Experiment 2 Larval Size: https://www.bco-dmo.org/dataset/725943
Phillips_2011 - Experiment 2 Larval Velum Size: https://www.bco-dmo.org/dataset/725957
Phillips_2011 - Experiment 2 Settlement Challenge 6: https://www.bco-dmo.org/dataset/725973
Phillips_2011 - Experiment 2 Settlement Challenge 8: https://www.bco-dmo.org/dataset/726002

Methods & Sampling

In this experiment, larval growth and metamorposis was tested using different food levels.

Larvae hatched on October 10, 2009 and ~50 were distributed into each tubs on 500mL filtered sea water (FSW). Because the greatest metamorphic success was in the Isochrysis galbana treatment during experiment 1, only that species was used in experiment 2.

Three food densities were created high food (4 x 104 cells mL-1 ), low food (4 x 103 cells mL-1 ) plus an Unfed treatment in which larvae were raised in FSW. Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. Each of the three treatments had three replicate containers; 50 larvae were placed into each container initially, exept two containers that had 1 extra. Every 3 days for 9 days starting on October 4, 2009, larvae were counted in each container to quantify survival. Water and food were also replenished. On days 6 and 8, fifteen larvae were transferred for the settlement challenge experiment.

Data Processing Description

For days 3 and 6: percent survival = (number alive on day of sampling/ number alive initial )*100
For day 9: percent survival = (number alive day 9/number remaining on day 8)*100

BCO-DMO Processing:

Added conventional header with dataset name, PI name, version date.
Modified parameter names to conform with BCO-DMO naming conventions.

Data Files

File
Phillips_2011_Expt2_LarvalMortality.csv (Comma Separated Values (.csv), 697 bytes) MD5:452f3fd69d6305e11b9f6f0c01b47f8a Primary data file for dataset ID 725880

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1130359

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Osenberg_et_al_Moorea		2003-05-19	Osenberg et al Moorea	Craig Osenberg (Principal Investigator)	data info

Instruments

Hemocytometer

Supplied Name: hemocytometer

Supplied Description:

Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment.

Instrument Type

Generic Name: Hemocytometer

Generic Description:

A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
FOOD_LEVEL	High (HI) Low (LO) or Unfed treatments	unitless	treatment
Replicate_tub	replicate number	unitless	replicate
number_alive_initial	the number larvae in treatments alive initially	unitless	count
number_alive_day_3	number of alive larvae found on day 3	unitless	count
number_dead_d3	number of dead larvae found on day 3	unitless	count
number_alive_d6	number of alive larvae found on day 6	unitless	count
number_dead_d6	number of dead larvae found on day 6	unitless	count
number_transferred_day_6	number of larvae transferred to settlement challenges	unitless	count
number_transferred_day_8	number of larvae transferred to settlement challenges on day 8	unitless	count
number_remain	the number of larvae still tubs after larvae for settlement challenges were removed	unitless	count
number_alive_d9	number of alive larvae found on day 9	unitless	count
number_dead_9	number of dead larvae found on day 9	unitless	count
percent_survival_day_3	survival from day 1 to 3	unitless (percent)	no_bcodmo_term
percent_survival_day_6	survival from day 3 to 6	unitless (percent)	no_bcodmo_term
percent_survival_day_9	survival from day 6 to 9	unitless (percent)	no_bcodmo_term

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Database

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Dataset: Phillips_2011 - Experiment 2 Larval Mortality