Dataset: Ross Sea metaproteome FASTA files
Deployment: NBP0601

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/769266

Principal Investigator:

Mak A. Saito (Woods Hole Oceanographic Institution, WHOI)

Contact:

Sara Bender (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Controls of Ross Sea Algal Community Structure (CORSACS)

Version:

Deployment Synonyms:

CORSACS I

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Description

A net tow metaproteome of a Phaeocystis antarctica bloom in the Ross Sea, mapped here to Phaeocystis metatranscriptomes analyzed by 2D LCMS, in units of protein spectral counts.

Related dataset (generated from the same sampling event and methodology):
* Ross Sea metaproteome protein spectral counts: https://www.bco-dmo.org/dataset/768237
* Ross Sea metaproteome peptide spectral counts: https://www.bco-dmo.org/dataset/768259

Methods & Sampling

Dataset acquisition description

Sampling was conducted with a 20 micron plankton net on 12/30/2005 at about 1am (local time), near station 137, was extracted for total protein using an SDS detergent method followed by tube gel purification, reduction, alkylation, and tryptic digestion and analyzed on a Thermo Orbitrap Fusion using 2-dimensional separation scheme to maximize metaproteome depth. Detailed methods available in Bender et al. 2018.

Data Processing Description

Dataset Processing Description

Mass spectra from 2D LC-MS was peptide-to-spectrum matched using the SEQUEST algorithm within Proteome Discoverer software, followed by spectral counting with Proteome Software's Scaffold software using the FASTA generated from Phaeocystis culture transcriptome study. Data processing documented in Bender et al. 2018.

More information about this dataset deployment

Funding

Award Number	Funding Source
OPP-0338097	NSF Office of Polar Programs (formerly NSF PLR)

Instruments

None available yet

Parameters

None available yet

Database

Contribute Data

Dataset: Ross Sea metaproteome FASTA files
Deployment: NBP0601

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Ross Sea metaproteome FASTA filesDeployment: NBP0601

Dataset acquisition description

Dataset Processing Description

Dataset: Ross Sea metaproteome FASTA files
Deployment: NBP0601