Dataset: P_Pools In Situ Experiments
Deployment: SAV-19-02

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/864291

Principal Investigator:

Solange Duhamel (Columbia University)

Co-Principal Investigator:

Julia Diaz (University of Georgia, UGA)

Contact:

Kahina Djaoudi (University of Arizona, UA)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)

Version:

Deployment Synonyms:

Zephyr, Zooming in on Enzymatic PhosphoHYdrolysis Reactions

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Description

Methods & Sampling

Dataset acquisition description

Sampling and analytical procedures:

Seawater samples were collected in triplicate from Niskin bottles (12 L), at three stations (St. 1, St. 2 and St. 3) along a transect from coastal Georgia to offshore waters. Latitude (lat) and longitude (lon) of sampling sites are provided in .mat files. Stations 1 and 2 were on the continental shelf (15–30 m depth), while Station 3 was on the shelf break (220 m depth) adjacent to the Gulf Stream. At each station, samples were collected at 2-3 depths between the surface (4–5 m) and 50 m for SRP, DOP and PP. SRP (50 mL), DOP (50 mL) and PP (2 L) were sampled from Niskin bottles. SRP and DOP samples were filtered through 0.2 µm polycarbonate filters and transferred into high density polyethylene bottles (HDPE, 60 mL) and kept frozen (-20°C) until analysis. PP samples were filtered through pre-combusted (450°C, 4.5h) and acid washed (1N HCl) GF/F filters, using a peristaltic pump. PP filters were wrapped in pre-combusted (450°C, 4.5h) aluminum foil, and kept frozen (-20°C) until analysis.

SRP, DOP and PP were analyzed following a spectrophotometric method based on the molybdenum reagent (Murphy and Riley, 1962). SRP concentrations were analyzed using the Liquid Waveguide Capillary Cell® (LWCC, optical length path = 2.5 m). DOP concentrations were determined by subtraction of SRP from the total dissolved phosphate (TDP). TDP was digested to SRP following the wet oxidation procedure (Pujo-Pay and Raimbault, 1994) and SRP was subsequently determined using the LWCC. PP concentrations were determined after high-temperature combustion (450°C, 4.5 h) and HCl extraction (0.5 N) (Strickland and Parsons, 1972). The released SRP following this procedure was then analyzed spectrophotometrically using a conventional spectrophotometer with a 10 cm cuvette.

Location: Northwestern Atlantic surface waters. Depth: surface-50 m.

Instruments: Sampling was performed using Niskin bottles (12 L) mounted on a rosette. Measurements of SRP, DOP were performed using the Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument). PP samples were measured using a Gynesis spectrophotometer.

Data Processing Description

Dataset Processing Description

Data were organized using MATLAB and output as .mat files. Gaps in data were filled with NaN in the .mat files.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1736967	NSF Division of Ocean Sciences
OCE-1737083	NSF Division of Ocean Sciences
OCE-1948042	NSF Division of Ocean Sciences
OCE-2001212	NSF Division of Ocean Sciences

Instruments

Liquid Waveguide Capillary Cells

Supplied Name: Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument).

Supplied Description:

Measurements of SRP, DOP were performed using the Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument).

Instrument Type

Generic Name: Liquid Waveguide Capillary Cells

Acronym: LWCC

Generic Description:

Liquid Waveguide Capillary Cells (LWCC) are optical sample cells that combine an increased optical pathlength (2-500 cm) with small sample volumes. They can be connected via optical fibers to a spectrophotometer with fiber optic capabilities. Similar to optical fibers, light is confined within the (liquid) core of an LWCC by total internal reflection at the core/wall interface. Ultra-sensitive absorbance measurements can be performed in the ultraviolet (UV), visible (VIS) and near-infrared (NIR) to detect low sample concentrations in a laboratory or process control environment. According to Beer’s Law the absorbance signal is proportional to chemical concentration and light path length.

Niskin bottle

Supplied Name:

Supplied Description:

Sampling was performed using Niskin bottles (12 L) mounted on a rosette.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Spectrophotometer

Supplied Name: Gynesis spectrophotometer

Supplied Description:

PP samples were measured using a Gynesis spectrophotometer.

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

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Database

Contribute Data

Dataset: P_Pools In Situ Experiments
Deployment: SAV-19-02

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: P_Pools In Situ ExperimentsDeployment: SAV-19-02

Dataset acquisition description

Dataset Processing Description

Dataset: P_Pools In Situ Experiments
Deployment: SAV-19-02