Dataset: IrnBru MV1405 - FvFm and Lifetime Fluorescence

Name: FvFm and fluorescence lifetime data collected from the R/V Melville MV1405 along the California Coastline during 2014
Published: 2016-07-21
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.652298.1

Spatial Extent: N:42.88 E:-117.62 S:32.81 W:-127.32

Temporal Extent: 2014-07-03 - 2014-07-25

Project:

Linking physiological and molecular aspects of diatom silicification in field populations (Diatom Silicification)

Principal Investigator:

Kimberlee Thamatrakoln (Rutgers University, Rutgers IMCS)

Co-Principal Investigator:

Mark A. Brzezinski (University of California-Santa Barbara, UCSB-LifeSci)

Scientist:

Dr Maxim Gorbunov (Rutgers University)

Dr Fedor Kuzminov (Rutgers University)

Contact:

Kimberlee Thamatrakoln (Rutgers University, Rutgers IMCS)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2016-07-21

Restricted:

Validated:

Yes

Current State:

Final no updates expected

FvFm and fluorescence lifetime data collected from the R/V Melville MV1405 along the California Coastline during 2014

Abstract:

FvFm and fluorescence lifetime data collected from the R/V Melville MV1405 along the California Coastline during 2014.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2016-07-21	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.652298.1	2020-01-29

Description

Quantum yield of photosynthesis (Fv/Fm) and lifetime fluorescence data from the MV1405 cruise (IrnBru).

[Lifetime fluorescence spectroscopy investigates the change in fluorescence over time of a sample when irradiated with UV, visible, or near-IR light. This decay in fluorescence can be measured over a wide time range: from picoseconds to milliseconds and beyond. - DMO] The average lifetime of these data are found in the TAV column.

Methods & Sampling

A custom-built Fast Repetition Rate Fluorometer and Lifetime Fluorometer measured surface water continuously using the ship's underway water system. Background fluorescence was measured daily using 0.2 um filtered seawater and manually subtracted from sample data.

Data Processing Description

For a complete description of data processing see Kuzminov and Gorbunov (2015)

BCO-DMO Processing Notes:
-filled in all blank cells with nd according to description in metadata
-changed column names to meet BCO-DMO naming standards
-removed header from file because repeated at toplevel
-separated the dateTime GMT column into two columns, date_gmt and time_gmt
-added a cruise_id and ISO_DateTime_UTC column

Data Files

File
FvFm_lifetime.csv (Comma Separated Values (.csv), 1.72 MB) MD5:b875ad8786155b3d6651cba47b0d5900 Primary data file for dataset ID 652298

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1333929
NSF Division of Ocean Sciences	OCE-1334387

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
MV1405	IrnBru IronBru	2014-07-03	R/V Melville	Kenneth W. Bruland (Chief Scientist)	data info

Instruments

Fast Repetition Rate Fluorometer

Supplied Description:

A custom-built Fast Repetition Rate Fluorometer continuously measured surface water using the ships underway water system.

Instrument Type

Generic Name: Fast Repetition Rate Fluorometer

Acronym: FRRf

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

An FRRf is used for measuring the fluorescence of a sample of phytoplankton photosynthetic competency (Fv/Fm).

Lifetime Fluorometer

Supplied Description:

A custom-built Lifetime Fluorometer continuously measured surface water using the ships underway water system.

Instrument Type

Generic Name: Lifetime Fluorometer

Generic Description:

A Lifetime Fluorometer is used to measure picosecond fluorescence decay kinetics in phytoplankton.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	cruise identification	unitless	cruise_id
date_gmt	GMT date; mm/dd/yy	unitless	date_gmt
time_gmt	GMT time; HH:MM	unitless	time_gmt
lat	latitude	decimal degrees	lat
lon	longitude	decimal degrees	lon
corr_Fluor_min	minimal fluorescence yield corrected for background fluorescence by subtracting respective value measured in 0.2 micrometers filtered seawater from sample data	relative units	fluor
corr_Fluor_max	maximal fluorescence yield corrected for background fluorescence by subtracting respective value measured in 0.2 micrometers filtered seawater from sample data	relative units	fluor
corr_FvFm	maximum photosynthetic efficiency of photosystem II (quantum yield of photochemistry)	dimensionless	Fv_Fm
functional_absorbtion	sigma; functional absorption cross-section of photosystem II (PSII)	unitless	no_bcodmo_term
NPQ	non-photochemical quenching	dimensionless	no_bcodmo_term
TAV	average lifetime of fluorescence	picosecond	mean
ISO_DateTime_UTC	DateTime (UTC) ISO formatted	unitless	ISO_DateTime_UTC
amp1	amplitude 1 of the different components of the deconvolution of the fluorescence lifetime kinetics	dimensionless	no_bcodmo_term
amp2	amplitude 2 of the different components of the deconvolution of the fluorescence lifetime kinetics	dimensionless	no_bcodmo_term
amp3	amplitude 3 of the different components of the deconvolution of the fluorescence lifetime kinetics	dimensionless	no_bcodmo_term
time1	time 1 of the different components of the fluorescence lifetime kinetics	picosecond	time_elapsed
time2	time 2 of the different components of the fluorescence lifetime kinetics	picosecond	time_elapsed
time3	time 3 of the different components of the fluorescence lifetime kinetics	picosecond	time_elapsed