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Series 1: Abiotic TEP Formation - DIC Perturbation
Results form a series of controlled laboratory experiments investigating the effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP).
The over determination columns (pCO2_pH_TA, pCO2_DIC_pH, pCO2_DIC_TA) give pCO2 (calculated using CO2Sys) for samples for which we measured three parameters, pH, total alkalinity (TA) and dissolved inorganic carbon (DIC). In all others we only measured pH and TA. These columns each give the calculated pCO2 in uatm, using pH and TA; DIC and pH and the DIC and TA respectively for calculations.
Related Files: Passow U (2012) The Abiotic Formation of TEP under Ocean Acidification Scenarios. Marine Chemistry 128-129:72-80
See: Passow U (2012) The Abiotic Formation of TEP under Ocean Acidification Scenarios. Marine Chemistry 128-129:72-80
Co2Sys was used to calculate the carbonate system parameter pCO2.
pCO2 calculated from total alkalinity, DIC and pH (total scale) using Co2Sys
BCO-DMO Processing Notes Original files: "Data.xls, Sheet: DICperturbation" and "Overcompensation.xls" contributed by Uta Passow - Approx Lat/Lon of Passow Lab appended to enable data discovery in MapServer - "nd" (no data) inserted into blank cells - Parameter names edited to conform to BCO-DMO parameter naming conventions
In brief, TA (μmol kg− 1) was measured within 4 hours after sampling by potentiometric titration (T50, Mettler Toledo) using certified acid titrant.
Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
Experiments 3 and 4, respectively, were conducted with 10 μm pre-filtered natural seawater and 10 μm pre-filtered sea water first incubated in 20 L bottles (bottle blooms).
A container, typically made of glass or plastic and with a narrow neck, used for storing drinks or other liquids.
Salinity was determined from TA samples using a conductivity instrument (3100 Yellow Springs Instruments).
Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.
A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.
Bottle blooms were incubated in clear 20 L bottles at 14 °C, at 15–30 μE s − 1 for 12:12 hours and bubbled with an aquarium pump during the light hours to keep the carbonate system stable.
A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
All experiments were conducted in rolling tanks at 14 °C in the dark.
Rolling tanks, which keep particles in suspension, thus simulating aggregate formation in situ.
Marine snow experiments are conducted in roller tanks, which turn continuously, keeping marine snow in suspension. It is important for marine snow not to touch surfaces. The rolling tanks, which keep particles in suspension, thus simulate aggregate formation in situ. Marine snow formation due to different types of oil was tested. Some treatments are easily identifiable as containing oil by their color (middle). UCSB, CA 2012.
The pHT (total scale) was measured with a spectrophotometer (Shimadzu Biospec 16-1) using the indicator dye mcresol purple (Sigma-Aldrich) within 1–2 hours after sampling.
An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
Chl. a was determined during growth of cultures and bottle blooms using a fluorometer (Turner 700) by filtration of samples onto 0.4 μm PC filters and soaking the filters in 90% acetone over night in the freezer (Strickland, 1972).
The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.
Lab Id – Lab identifier where experiments were conducted
Approximate Latitude Position of Lab; South is negative
Approximate Longitude Position of Lab; West is negative
Experiment Number
Experiment Date Range
Substrate
Treatments
Replicates
pCO2 at T0
pCO2 at T24
pCO2 pH TA
pCO2 DIC pH
pCO2 DIC TA
pH at T0
pH at T24
TEP at T0 in ug of Gxeq (xanthan gum weight equivalent) per liter
TEP at T24 in ug of Gxeq (xanthan gum weight equivalent) per liter
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