Dataset: Thaps_57Fe_distribution
Deployment: lab_Kustka

Fe limited diatoms were supplied with excess 57Fe and the new Fe was tracked to better understand storage mechanisms.

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/516730

Lead Principal Investigator:

Adam Kustka (Rutgers University)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Iron storage in diatoms and N2 fixing cyanobacteria: mechanisms, regulation and biogeochemical significance (Mechanisms of Fe storage in phytoplankton)

Current State:

Restricted

Version:

13 June 2014

Version Date:

2014-06-13

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Description

Status (June 2014): Data and metadata have been contributed to BCO-DMO. Data are restricted until April 2015. Manuscript submission is expected during the fall 2014.

Data contributed include 3 Excel files of ion intensities of 12C, 28Si, 31P, 56Fe, 40Ca and 57Fe within each region of interest as well as the Mathematica script written by A. Kustka used to conduct the Monte Carlo permutation.

Methods & Sampling

Dataset acquisition description

Experiments were conducted to probe the intracellular iron (Fe) distribution in Thalassiosira pseudonana. These experiments can be summarized as follows:

Three cells of T. pseudonana were analyzed for relative elemental contents at nanometer scale resolution using NanoSIMS mass spectrometry. These diatoms were first grown under low Fe reflecting natural abundance iron isotopic ratios and then spiked with excessive concentrations of Fe heavily enriched in 57Fe, in order to track the fate of newly incorporated 57Fe relative to pre-existing pools of 56Fe. The spatial distribution of this newly incorporated iron was evaluated using Geary's C statistic of spatial correlation, and significant deviations from random were established by a Monte Carlo permutation experiment.

Data Processing Description

Dataset Processing Description

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0727889	NSF Division of Ocean Sciences

Instruments

Mass Spectrometer

Supplied Name: NanoSIMS mass spectrometry

Supplied Description:

Three cells of T. pseudonana were analyzed for relative elemental contents at nanometer scale resolution using Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS). Secondary Ion Mass Spectrometry (SIMS) is a surface analysis technique that provides information about the lateral distribution of any element and its isotopes, and quantitative information about the isotopic composition of a sample. NanoSIMS allows for elemental and isotopic analysis down to the 50-nanomenter scale.

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
diatom	Number identifying the diatom studied.	integer	individual
ROI_num	Identification number for the region of interest (ROI).	integer	no_bcodmo_term
C12	Ion intensity of Carbon-12 (12C).	dimensionless	no_bcodmo_term
Si28	Ion intensity of Silicon-28 (28Si).	dimensionless	no_bcodmo_term
P31	Ion intensity of Phosphorus-31 (31P).	dimensionless	no_bcodmo_term
Fe56	Ion intensity of Iron-56 (56Fe).	dimensionless	no_bcodmo_term
Ca40	Ion intensity of Calcium-40 (40Ca).	dimensionless	no_bcodmo_term
Fe57	Ion intensity of Iron-57 (57Fe).	dimensionless	no_bcodmo_term
ROI_area	Area of the region of interest (ROI).	microns^2	no_bcodmo_term
ROI_diam	Diamter of the region of interest (ROI).	microns	no_bcodmo_term
ROI_X	?	?	no_bcodmo_term
ROI_Y	?	?	no_bcodmo_term
ROI_XSTG	X stage coordinate on the nanoSIMS microscope.	dimensionless	no_bcodmo_term
ROI_YSTG	Y stage coordinate on the nanoSIMS microscope.	dimensionless	no_bcodmo_term

Database

Contribute Data

Dataset: Thaps_57Fe_distribution
Deployment: lab_Kustka

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Thaps_57Fe_distributionDeployment: lab_Kustka

Dataset acquisition description

Dataset Processing Description

Dataset: Thaps_57Fe_distribution
Deployment: lab_Kustka