Dataset: BF In Situ Experiments
Deployment: SAV-19-02

View Data: For data, See Dataset Metadata Page: https://osprey.bco-dmo.org/dataset/864280

Principal Investigator:

Solange Duhamel (Columbia University)

Co-Principal Investigator:

Julia Diaz (University of Georgia, UGA)

Contact:

Kahina Djaoudi (University of Arizona, UA)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)

Version:

Deployment Synonyms:

Zephyr, Zooming in on Enzymatic PhosphoHYdrolysis Reactions

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Description

Methods & Sampling

Dataset acquisition description

Sampling and analytical procedures:

Seawater samples for bioavailability factor determinations were collected from Niskin bottles (12 L), at station 1 (two measurements) and 3 (one measurement) along a transect from coastal Georgia to offshore waters. The bioavailability factor was calculated based on changes in PO43- turnover time. The bioavailability factor (BF) is calculated as follows: BF=TE-TN/ TP-TN (Björkman and Karl, 1994), where TE reflects PO43- turnover time in the DOP amended treatment, TN the PO43- turnover time in the control (no additions) and TP the PO43- turnover time in the treatment amended with Pi. In this study, treatments were amended with polyphosphate (Polyp), adenosine triphosphate (ATP), adenosine monophosphate (AMP) or methyl phosphonate (Mepn).

BF ranges from 0 for an unavailable substrate, to a value of 1 for a DOP model substrate having a bioavailability equal to that of +Pi.

Instruments: Sampling was performed using Niskin bottles (12 L) mounted on a rosette. Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.

Location: Northwestern Atlantic surface waters. Depth: surface-50 m.

Problem report: BF in station 1 (first determination) were determined in duplicates instead of triplicate in station 1 (second determination) and station 3. Therefore, gaps are filled with NaN in the .mat files.

Data Processing Description

Dataset Processing Description

Data were organized using MATLAB and output as .mat files. Gaps in data were filled with NaN in the .mat files.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1736967	NSF Division of Ocean Sciences
OCE-1737083	NSF Division of Ocean Sciences
OCE-1948042	NSF Division of Ocean Sciences
OCE-2001212	NSF Division of Ocean Sciences

Instruments

Liquid Scintillation Counter

Supplied Name:

Supplied Description:

Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.

Instrument Type

Generic Name: Liquid Scintillation Counter

Acronym: LSC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Generic Description:

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Niskin bottle

Supplied Name:

Supplied Description:

Sampling was performed using Niskin bottles (12 L) mounted on a rosette.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

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Database

Contribute Data

Dataset: BF In Situ Experiments
Deployment: SAV-19-02

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: BF In Situ ExperimentsDeployment: SAV-19-02

Dataset acquisition description

Dataset Processing Description

Dataset: BF In Situ Experiments
Deployment: SAV-19-02