Dataset: WaterChemistry_OA_Expt2017

To assess the interactive effects of temperature and pCO2 on Dunaliella excentricus feeding behavior, we reared the larvae (2 individuals mL⁻¹) in six treatment conditions. The treatment conditions combined three levels of pCO2 bubbled into individual 3-L jars: 400 ppm (ambient atmospheric level), 800 ppm (moderate atmospheric level), and 1,500 ppm (high atmospheric level), and two temperatures maintained in incubators: 12°C (ambient) and 17°C (high). We also maintained pre-equilibrated ambient, moderate, and high pCO2 treatment water in tanks within the same incubators as the treatment jars for periodic water changes. We measured the water chemistry of each treatment jar and tank over the course of the experiment.

DIC samples were gently filtered through a GFF syringe filter, stored in airtight vials, and analyzed on the same day with an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer. Measures were then corrected for density based on temperature and salinity. pH samples were also gently filtered through a GFF syringe filter, stored in airtight vials, and analyzed on the same day using a spectrophotometric method. Samples were placed in a temperature-controlled water bath to reach 25°C, carefully transferred to jacketed 5-cm cuvettes, and analyzed with a spectrophotometer. An absorbance measurement was taken after the addition of 20-uL of m-cresol blue to compare with a baseline measurement to determine the pH of each sample. The ratio of the two absorbance peaks was corrected based on samples with two dye additions.

This dataset includes unprocessed data.