Dataset: OFP Lipid Flux

Name: Fluxes of lipid biomarkers from the Oceanic Flux Program sediment trap time-series in the North Sargasso Sea from September to December 2016
Published: 2019-09-04
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.775902.1

Spatial Extent: N:31.8333 E:-64.1666 S:31.8333 W:-64.1666

Temporal Extent: 2016-09-25 - 2016-12-10

Project:

Time Series Particle Flux Measurements in the Sargasso Sea (OFP Sargasso Sea)

Program:

Oceanic Flux Program (OFP)

Principal Investigator:

Maureen H. Conte (Bermuda Institute of Ocean Sciences, BIOS)

Scientist:

Rut Pedrosa Pàmies (Marine Biological Laboratory Ecosystems Center, MBL - Ecosystems)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-09-04

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Fluxes of lipid biomarkers from the Oceanic Flux Program sediment trap time-series in the North Sargasso Sea from September to December 2016

Abstract:

Fluxes of lipid biomarkers in the 1500 m and 3200 m Oceanic Flux Program sediment traps in the northern Sargasso Sea, before, during and after of Hurricane Nicole 2016 (Sep-Dec 2016)

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2019-09-04	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.775902.1	2019-09-04

Description

Fluxes of lipid biomarkers in the 1500 m and 3200 m Oceanic Flux Program sediment traps in the northern Sargasso Sea, before, during and after of Hurricane Nicole 2016 (Sep-Dec 2016)

Methods & Sampling

Sinking particles were collected at 1500 m and 3200 m depths by Parflux sediment traps (McLane Labs, Falmouth, MA) on the Oceanic Flux Program (OFP) mooring. Trap cups were filled with 40 ppt deep seawater (concentrated by freezing) poisoned with ultra-trace metal purity HgCl2 (200 mg L-1). OFP methods are detailed in Conte et al. (2001, 2018). Sinking particles analyzed in this study were collected between 25 October 2016 and 10 December 2016 with a biweekly sample resolution.

Lipids were preserved and analyzed using a modification of methods in Conte et al. (2003) (Pedrosa-Pàmies et al., 2018). Briefly, an internal standard mixture (n-C21:0 fatty alcohol, n-C23:0 fatty acid, 5α-cholestane and n-C36:0 alkane) was added to the samples prior to lipid extraction. Lipids were ultrasonically extracted in 2:1 CHCl3-MeOH, transesterified with methanolic HCl and trimethylsilylated using BSTFA with 1% TMCS. The transesterified, trimethylsilyl derivatives were analyzed on an Agilent 7890A GC coupled to a 5975C MS. Compounds were identified by mass spectra and quantified from their FID response relative to the internal standard.

Data Processing Description

Lipid compounds were identified by their mass spectra and were quantified from their FID response relative to the internal standard. The software used were MSD ChemStation (version E.0202.1431) and Chrom Perfect (version 5.5.2).

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- re-formatted date from d-Mon-yy to yyyy-mm-dd

Data Files

File
lipid_flux.csv (Comma Separated Values (.csv), 2.65 KB) MD5:88c11d1b9be8288f20b1bea37f829261 Primary data file for dataset ID 775902

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1536644

Deployments

None available yet

Instruments

Mass Spectrometer

Supplied Name: Gas chromatograph-mass spectrometer

Supplied Description:

Gas chromatograph-mass spectrometer (GC-MS): Agilent 7890A GC coupled to a 5975C MS equipped with triple-axis MS and FID detectors.

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
depth_m	depth of observation	meters (m)	depth
start_date	start date in yyyy-mm-dd format	unitless	start_date
end_date	end date in yyyy-mm-dd format	unitless	end_date
days_sampling	number of days sampling	days	days
poc_perc	Particulate Organic Carbon percentage of total mass flux	unitless	POC
poc_flux	Particulate Organic Carbon flux	milligrams per meter squared per day (mg/m2/d)	POC
total_lipid_perc	Total extractable lipids percentage of total POC	unitless	lipids_tot
total_lipid_flux	Total extractable lipids flux	micrograms per meter squared per day (ug/m2/d)	lipids_tot
tfa_flux	Total fatty acids flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
sfa_flux	C12-C28 saturated even fatty acids flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
mufa_flux	C14:1-C22:1 monosaturated even fatty acids flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_18_1w9_fa	18:1ω9 fatty acid flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_18_1w7_fa	18:1ω7 fatty acid flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
pufas_flux	C16-C20 polyunsaturated fatty acids flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_18_3w6_pufa	18:3ω6 polyunsaturated fatty acid flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_20_5w3_pufa	20:5ω3 polyunsaturated fatty acid flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_22_6w3_pufa	22:6ω3 polyunsaturated fatty acid flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
odd_br_fa_flux	C13-C19 odd and saturated iso- anteiso-branched fatty acid flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
tfal_flux	Total Fatty alcohols flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
mufal_flux	C16-22 Monounsaturated fatty alcohols	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
odd_br_fal_flux	C11-21 Odd and iso- anteiso-branched fatty alcohols flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
tbeta_omega_1_hydroxyacids	+ω-1 hydroxyacids flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
tsterols_stanols	Total sterols+stanols flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
phytosterols	Phytosterols= (24-Nor-cholesta-5; 22-dien-3β-ol (C26Δ5;22); 27-nor-24-methylcholesta-5;22-dien-3β-ol (Cnor27Δ5;22); Cholesta-5;22-dien-3β -ol (C27Δ5;22); 24-Methylcholesta-5;22-dien-3β-ol (C28Δ5;22); 24-Methylcholesta-5;24(28)-dien-3β-ol (C28Δ5;24(28)); 24-Methylcholest-5-en-3β-ol (C28Δ5); 24-Ethylcholesta-5;22-dien-3β-ol (C29Δ5;22); 24-Ethylcholest-5-en-3β-ol (C29Δ5); 24-Propylcholesta-5;24(28)-dien-3β-ol (C30Δ5;24(28)); 4α;23;24-Trimethyl-5a-cholest-22-en-3β-ol (4αC30Δ22) ) flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
nor27delta5_22e	Cholesta-5;22-dien-3β -ol (Occelasterol) flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_27delta5_22e	Cholesta-5;22-dien-3β -ol (Dehidrocholesterol) flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_28delta5_22e	24-Methylcholesta-5;22-dien-3β-ol ( Brassicasterol) flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_29delta5_22e	24-Ethylcholesta-5;22-dien-3β-ol (Estigmasterol) flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_27delta5	Cholest-5-en-3β-ol (cholesterol) flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_28delta5	4-Methylcholest-5-en-3β-ol flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_29delta5	24-Ethylcholest-5-en-3β-ol flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
tstanols	Stanols= ( 24-Nor-5α-cholest-22-en-3β-ol (C26Δ22); 5α-Cholesta-22-en-3β-ol (C27Δ22); 24-Methyl-5α-cholest-22-en-3β-ol (C28Δ22); 24-Ehtyl-colesta-22-en-3β-ol (C29Δ22); 5α-Cholestan-3β-ol (C27Δ0); 24-Methyl-colestan-3β-ol (C28Δ0); 24-Ethyl-5α-cholestan-3β-ol (C29Δ0); 4α;23S;24R-trimethyl-5α(H)-cholestan-3β-ol (4αC30Δ0).	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_27delta0	5α-Cholestan-3β-ol) flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_28delta0	24-Methyl-colestan-3β-ol flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_29delta0	24-Ethyl-5α-cholestan-3β-ol flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_27delta22	5α-Cholesta-22-en-3β-ol flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
sk	Saturated 4-methyl steroidal ketones flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
c30diol	C30 alkan-1;15-diol flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
hop	Hopanoids flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
flux_1_o_alkyl	1-O-alkylglycerols flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term
alkenones	C37-C39 methyl ketones flux	micrograms per meter squared per day (ug/m2/d)	no_bcodmo_term

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